Purified Mouse Anti-Human CD3
Clone SP34-2 (RUO)
- Brand BD Pharmingen™
- Alternative Name CD3E; CD3-epsilon; T3E; TCRE
- Concentration 0.5 mg/ml
- Isotype Mouse BALB/c IgG1, λ
- Reactivity Human (QC Testing) Rhesus, Baboon, Cynomolgus (Tested in Development)
Flow cytometry (Routinely Tested)
- Immunogen Purified Human CD3ε Protein
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
Clone SP34-2 is a mouse IgG1 isotype monoclonal antibody, descendant of SP34 (mouse IgG3), with the same specificity and reactivity pattern as the parent clone. It cross-reacts with a major subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, and rhesus, cynomolgus, and pigtail macaque monkeys. The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes with the majority of CD3-positive cells being negative when dual stained with antibodies to B or NK cells markers. SP34-2 is also capable of inducing cell proliferation on both human and non-human primate PBMC.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Species testing during development may have been performed with a different format of the same clone. Selected applications have been tested for cross-reactivity.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Clone SP34-2 is routinely tested using Purified Mouse IgG1 κ , clone MOPC-21 (Cat. No. Cat. No 556648 ), as the isotype control. Alternate isotype controls specific for the lambda light chain, such as clones S1-68.1 (Cat No. 553452) and A111-3 (Cat. No. 553485), are not routinely tested for flow cytometry application. Investigators are encouraged to validate the alternative clones for the desired applications.