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BUV737 Mouse Anti-Human CD64 10.1 RUO

BUV737 Mouse Anti-Human CD64 

Clone  10.1   (RUO)

  • Product Announcement

    This product will be discontinued in July.
    612777 is the replacement for 564426
    Click here for more information

    Product Announcement

    Why is this product being discontinued?

    The tandem dye was reformulated. The original version of the dye will be transitioned to the new version of the dye. The current off-the-shelf catalog number will be discontinued and replaced with a new off-the-shelf catalog number.

    What is the difference between 564426 and 612777?

    The tandem dye was reformulated. However, both 564426 and 612777 have very similar excitation and emission spectra and are excited by the same laser line. So you don�t need to change your filters or instrument configuration; you also don�t need to change your panel design.

    Does the new formulation of the tandem dye have less spillover?

    For some reformulated BUV tandem dyes there may be less spillover into the BUV395 detector, so we recommend resetting your compensation values when using the new version of the dye. You also get better long-term performance stability with the new formulation.

    When will this product be discontinued?

    564426 will be discontinued in July 2019. 612777 is a replacement for 564426 and is currently available for purchase.

    How will I know if I have the original formulation or the new formulation of the tandem dye?

    BUV tandem dyes made with the new formulation have a catalog number starting with 612777 and are packaged in black vials. BUV tandem dyes made with the original formulation have a catalog number starting with 564426 and will remain amber vials.

    Why was the BUV tandem dye reformulated?

    The BUV tandem dyes were redeveloped to be compatible with BD OptiBuild� custom technology. We can now offer more BUV tandem fluorochrome options with the antibodies you need, including more esoteric clone/dye combinations.

    Click here for more information

  • Brand BD Horizon™
  • Alternative Name FCGR1; FcRI; Fc-gamma RI; IgG Fc Receptor I; High affinity IgG FcR1
  • Vol. Per Test 5 µl
  • Isotype Mouse BALB/c IgG1, κ
  • Reactivity Human (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Human Rheumatoid synovial fluid cells and fibronectin-purified monocytes
  • Workshop No. VI MA36
  • Entrez Gene ID 2209 
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Detalhes do produto Recommended Assay Referências

Description

The 10.1 monoclonal antibody specifically binds to CD64, a 72 kDa type I transmembrane glycoprotein that is a high affinity receptor for human IgG (FcγRI), especially the IgG1 and IgG3 subclasses. CD64 is expressed on monocytes, macrophages, dendritic cells, granulocytes activated with interferon-gamma and early myeloid lineage cells. CD64 associates with a signaling FcRγ homodimer to form the functional high affinity FcγRI complex. CD64 functions in both innate and adaptive immune responses and mediates endocytosis, phagocytosis, antigen presentation, antibody-dependent cellular toxicity, cytokine release and superoxide generation.

The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor® 700-like dyes (e.g., 712/20-nm filter).

Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV737 reagents (e.g., CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.

FORMATO
  • Format BUV737
  • Excitation Source Ultraviolet 355 nm
  • Excitation Max 348 nm
  • Emission Max 737 nm

BUV737 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an Em Max at 737 nm. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover in to channels detecting Alexa Fluor® 700 like dyes (for example, 712/20-nm filter). BUV737 has been exclusively developed by BD Biosciences for instruments equipped with a 355-nm UV laser.

Other Formats →

Suggested Companion Products

Stain Buffer (FBS) RUO
500 mL Cat No: 554656

Stain Buffer (BSA) RUO
500 mL Cat No: 554657

BUV737 Mouse IgG1, κ Isotype Control RUO
50 µg Cat No: 564299

BUV737 Mouse Anti-Human CD64 10.1 RUO
25 Tests Cat No: 564426

Lysing Solution 10X Concentrate IVD
100 Cat No: 349202

Lysing Buffer RUO
100 mL Cat No: 555899

Brilliant Stain Buffer RUO
100 Tests Cat No: 563794

RECURSOS E FERRAMENTAS
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Download TDS
Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV737 under optimum conditions, and unconjugated antibody and free BD Horizon BUV737 were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.


  1. CD64. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007; :151.

  2. Dougherty GJ, Selvendran Y, Murdoch S, Palmer DG, Hogg N. The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1. Eur J Immunol. 1987; 17(10):1453-1459. View reference

  3. Indik ZK, Hunter S, Huang MM, et al. The high affinity Fc gamma receptor (CD64) induces phagocytosis in the absence of its cytoplasmic domain: the gamma subunit of Fc gamma RIIIA imparts phagocytic function to Fc gamma RI. Exp Hematol. 1994; 22(7):599-606. View reference

  4. Sun W, O'Shea JJ, Guyre PM. CD64 Workshop Panel Report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997; :988-990.

  5. van Vugt MJ, Heijnen AF, Capel PJ, et al. FcR gamma-chain is essential for both surface expression and function of human Fc gamma RI (CD64) in vivo. Blood. 1996; 87(9):3593-3599. View reference

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