- Brand BD Pharmingen™
Immunohistochemistry (Routinely Tested)
- Storage Buffer Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.
- Regulatory Status RUO
Regulatory Status Legend
Streptavidin-Horseradish peroxidase (SAv-HRP) is a useful detection enzyme for labeling biotinylated-primary or seconday antibodies for detection by immunohistochemistry. The streptavidin has very high affinity for biotin conjugated to the antibody and the horseradish peroxidase serves as the enzyme to change color in different substrates. This pre-diluted SAv-HRP is convenient to use and provides optimal signal amplification and detection.
This product is routinely tested by immunohistochemistry. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Streptavidin was conjugated with dye under optimum conditions, and unconjugated Streptavidin and free dye were removed
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- ProClin is a trademark of Rohm and Haas Company.
- Hazardous Ingredient: ProClin™ 150. Avoid exposure to skin and eyes and ingestion. Wash exposed skin with soap and water. Flush eyes with water.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Immunohistochemistry: SAv-HRP is ready to use in its pre-diluted format. Remove the solution from 4°C and let it equilibrate to room temperature before using. The SAv-HRP should be used after the slides are incubated with biotinylated primary or secondary antibody and the unbound antibody is washed away. Apply enough SAv-HRP to cover the tissue sections and incubate 30 minutes at room temperature. Wash 3X with PBS and apply the appropriate substrate for horseradish peroxidase (inquire with technical service for available substrates from BD Pharmingen™).
Note: It is important not to use Azide in any of the buffers when using horseradish peroxidase as azide inactivates the enzyme.