CD45 V500-C

BD Horizon™ CD45 V500-C

Name: CD45 V500-C
Brand: BD Horizon™
SKU: 647450

Clone 2D1

(ASR)

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Product Details

Brand: BD Horizon™

Alternative Name: PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5

Reactivity: Human

Isotype: Mouse IgG1, κ

Application: Flow cytometry

Concentration: 100 μg/mL

Vol. Per Test: 5 μL

Storage Buffer: Phosphate buffered saline with BSA and ProClin®.

Regulatory Status: ASR

Preparation And Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

647450 Rev. 1

Antibody Details

2D1

The CD45 antibody, clone 2D1, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood mononuclear cells (PBMCs).

The CD45 antibody (anti–HLe-1) recognizes members of the T200 family of human leucocyte antigens with molecular mass of 180 to 220 kilodaltons (kDa.)

647450 Rev. 1

Format Details

V500

BD Horizon™ V500 Dye is part of the BD Horizon™ Violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 415-nm and an emission maximum (Em Max) at 499-nm. Driven by BD innovation, V500 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 500 nm (eg, a 525/50 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: V500

Excitation Source: Violet 405 nm

Excitation Max: 415 nm

Emission Max: 499 nm

647450 Rev.1

Citations & References

Development References (4)

Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Jackson A. Basic phenotyping of lymphocytes: selection and testing of reagents and interpretation of data. Clin Immunol Newslett. 1990; 10:43-55. (Biology).
Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.

View All (4)

647450 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

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