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PE Armenian Hamster Anti-ICOS (CD278)
PE Armenian Hamster Anti-ICOS (CD278)
Analysis of ICOS (CD278) Expression     Left Panel - Two-color flow cytometric analysis of ICOS (CD278) expression on human peripheral blood mononuclear cells. Human PBMC were stained with APC Mouse Anti-Human CD3 antibody (Cat. No. 555335/561810/561811) and either no antibody (PE Autofluorescence Control; Top Plot) or PE Hamster Anti-ICOS (CD278) antibody (Cat. No. 565669; Bottom Plot). Two-color flow cytometric contour plots showing the correlated expression of ICOS (CD278) [or PE Autofluorescence] versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.     Right Panel - Multicolor flow cytometric analysis of ICOS (CD278) expression on mouse thymocytes. Mouse thymocytes were stained with BD Horizon™ V450 Rat anti-Mouse CD4 (Cat. No. 560468/560470) and APC-H7 Rat anti-Mouse CD8a (Cat. No. 560182/560247) antibodies, and either no antibody (PE Autofluorescence Control; dashed line histograms) or PE Hamster Anti-ICOS (CD278) antibody (solid line histograms).  The fluorescence histograms showing ICOS (CD278) expression (or PE Autofluorescence) were derived from CD4 and CD8 gated events with the forward and side light-scatter characteristics of viable thymocytes as indicated.     Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Analysis of ICOS (CD278) Expression     Left Panel - Two-color flow cytometric analysis of ICOS (CD278) expression on human peripheral blood mononuclear cells. Human PBMC were stained with APC Mouse Anti-Human CD3 antibody (Cat. No. 555335/561810/561811) and either no antibody (PE Autofluorescence Control; Top Plot) or PE Hamster Anti-ICOS (CD278) antibody (Cat. No. 565669; Bottom Plot). Two-color flow cytometric contour plots showing the correlated expression of ICOS (CD278) [or PE Autofluorescence] versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.     Right Panel - Multicolor flow cytometric analysis of ICOS (CD278) expression on mouse thymocytes. Mouse thymocytes were stained with BD Horizon™ V450 Rat anti-Mouse CD4 (Cat. No. 560468/560470) and APC-H7 Rat anti-Mouse CD8a (Cat. No. 560182/560247) antibodies, and either no antibody (PE Autofluorescence Control; dashed line histograms) or PE Hamster Anti-ICOS (CD278) antibody (solid line histograms).  The fluorescence histograms showing ICOS (CD278) expression (or PE Autofluorescence) were derived from CD4 and CD8 gated events with the forward and side light-scatter characteristics of viable thymocytes as indicated.     Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Product Details
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BD Pharmingen™
ICOS; AILIM; H4; Inducible T-cell costimulator; CVID1; Ly115
Human (QC Testing), Mouse (Tested in Development), Rat, Rhesus (Reported)
Armenian Hamster IgG
Mouse D10.G4.1 T-cell clone
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739326
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. An isotype control should be used at the same concentration as the antibody of interest.
565669 Rev. 2
Antibody Details
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C398.4A

The C398.4A monoclonal antibody specifically binds to Inducible Costimulator (ICOS), which is also known as, CD278, Activation-inducible lymphocyte immunomediatory molecule (AILIM), or H4. ICOS is a type I transmembrane glycoprotein that forms a disulfide-linked homodimer and belongs to the CD28 family within the Ig superfamily. ICOS is expressed on either CD4+ or CD8+ single-positive mature thymocytes, T cells, or subsets of Innate Lymphoid Cells (ILC). Its expression is upregulated on activated T lymphocytes. ICOS is a costimulatory receptor that can bind to the B7-H2 ligand (CD275, ICOS-L) that is expressed on B cells, monocytes, macrophages, dendritic cells, and endothelial cells. ICOS plays a critical role in many types of T cell-dependent immunity. In the case of humoral immunity, for example, ICOS signaling is critical for the differentiation of T follicular helper (Tfh) cells and development of germinal centers. Although C398.4A was generated against mouse ICOS, this antibody reportedly crossreacts with human, rhesus, and rat ICOS.

565669 Rev. 2
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565669 Rev.2
Citations & References
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Development References (11)

  1. Araujo LM, Fert I, Jouhault Q, et al. Increased production of interleukin-17 over interleukin-10 by treg cells implicates inducible costimulator molecule in experimental spondyloarthritis. Arthritis Rheum. 2014; 66(9):2412-2422. (Clone-specific: Flow cytometry). View Reference
  2. Brenchley JM, Vinton C, Tabb B, et al. Differential infection patterns of CD4+ T cells and lymphoid tissue viral burden distinguish progressive and nonprogressive lentiviral infections.. Blood. 2012; 120(20):4172-81. (Clone-specific: Flow cytometry). View Reference
  3. Buonfiglio D, Bragardo M, Bonissoni S, et al. Characterization of a novel human surface molecule selectively expressed by mature thymocytes, activated T cells and subsets of T cell lymphomas. Eur J Immunol. 1999; 29(9):2863-2879. (Clone-specific: Flow cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay). View Reference
  4. Buonfiglio D, Bragardo M, Redoglia V, et al. The T cell activation molecule H4 and the CD28-like molecule ICOS are identical. Eur J Immunol. 2000; 30(12):3463-3467. (Clone-specific: Blocking, Flow cytometry). View Reference
  5. Chen Y, Shen S, Gorentla BK, Gao J, Zhong XP. Murine regulatory T cells contain hyperproliferative and death-prone subsets with differential ICOS expression. J Immunol. 2012; 188(4):1698-1707. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
  6. Hutloff A, Dittrich AM, Beier KC, et al. ICOS is an inducible T-cell co-stimulator structurally and functionally related to CD28. Nature. 1999; 397(3716):263-266. (Biology). View Reference
  7. Klatt NR, Vinton CL, Lynch RM et al. SIV infection of rhesus macaques results in dysfunctional T- and B-cell responses to neo and recall Leishmania major vaccination. Blood. 2011; 118(22):5803-5812. (Clone-specific: Flow cytometry). View Reference
  8. McAdam AJ, Chang TT, Lumelsky AE, et al. Mouse inducible costimulatory molecule (ICOS) expression is enhanced by CD28 costimulation and regulates differentiation of CD4+ T cells.. J Immunol. 2000; 165(9):5035-40. (Biology). View Reference
  9. Redoglia V, Dianzani U, Rojo JM, et al. Characterization of H4: a mouse T lymphocyte activation molecule functionally associated with the CD3/T cell receptor. Eur J Immunol. 1996; 26(11):2781-2789. (Immunogen: Bioassay, (Co)-stimulation, Flow cytometry, Functional assay, Immunoprecipitation, Radioimmunoassay). View Reference
  10. Sonnenberg GF, Mjosberg J, Spits H, Artis D. SnapShot: innate lymphoid cells. Immunity. 2013; 39(3):622-623. (Biology). View Reference
  11. Xu H, Wang X, Lackner AA, Veazey RS. PD-1(HIGH) Follicular CD4 T Helper Cell Subsets Residing in Lymph Node Germinal Centers Correlate with B Cell Maturation and IgG Production in Rhesus Macaques. Front Biosci. 2014; 5(85):1-7. (Clone-specific: Flow cytometry). View Reference
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565669 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.