Flow cytometric analysis of CD32 on rat splenocytes. Lewis rat splenocytes were simultaneously stained with a FITC Mouse Anti-Rat IgM antibody (Cat. No. 553887) and a PE Mouse Anti-Rat CD32 antibody (Cat. No. 562189). A flow cytometric fluorescence dot plot showing CD32 versus IgM expression were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
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Flow cytometric analysis of CD32 on rat splenocytes. Lewis rat splenocytes were simultaneously stained with a FITC Mouse Anti-Rat IgM antibody (Cat. No. 553887) and a PE Mouse Anti-Rat CD32 antibody (Cat. No. 562189). A flow cytometric fluorescence dot plot showing CD32 versus IgM expression were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
Fcgr2b; FcRII; Fc-gamma RII; FcγRII; FcγII receptor
Rat (QC Testing)
Mouse BALB/c IgG1, κ
Recombinant Rat CD32 Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_11153308
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
550617
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FITC Mouse Anti-Rat IgM RUO
Size
0.5 mg
Cat No.
553887
562189 Rev. 1
Antibody Details
D34-485
The D34-485 monoclonal antibody specifically recognizes CD32, the FcγII receptor. Rat CD32 is expressed on B lymphocytes, myeloid cells, and some lymphocytes in the thymic medulla. D34-485 antibody blocks binding of aggregated immunoglobulins to the FcγII receptors in vitro.
562189 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
562189 Rev.1
Citations & References
Development References (2)
-
Mara-Koosham G, Hutt JA, Lyons CR, Wu TH. Antibodies contribute to effective vaccination against respiratory infection by type A Francisella tularensis strains. Infect Immun. 2011; 79(4):1770-1778. (Clone-specific: Blocking). View Reference
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Yrlid U, Cerovic V, Milling S, Jenkins CD, Klavinskis LS, MacPherson GG. A distinct subset of intestinal dendritic cells responds selectively to oral TLR7/8 stimulation. Eur J Immunol. 2006; 36(10):2639-2648. (Clone-specific: Flow cytometry). View Reference
562189 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
