Flow cytometric analysis of PE-conjugated anti-mouse CD229.1 on mouse splenocytes. Splenocytes from BALB/c mice were stained with PE anti-mouse CD229.1 (clone 30C7, Cat. No. 560201) and compared to splenocytes stained with a PE rat IgG2a isotype control (clone R35-95, Cat. No. 553930). The isotype control is represented by a dashed line and the PE anti-mouse CD229.1 by the solid line. Flow cytometry was performed on a BD FACSCalibur™ System.
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Flow cytometric analysis of PE-conjugated anti-mouse CD229.1 on mouse splenocytes. Splenocytes from BALB/c mice were stained with PE anti-mouse CD229.1 (clone 30C7, Cat. No. 560201) and compared to splenocytes stained with a PE rat IgG2a isotype control (clone R35-95, Cat. No. 553930). The isotype control is represented by a dashed line and the PE anti-mouse CD229.1 by the solid line. Flow cytometry was performed on a BD FACSCalibur™ System.
Product Details
BD Pharmingen™
Ly-9.1; T100; CD229; Lgp100; SLAMF3
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
SJL/J mouse splenocytes
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_1645249
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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560201 Rev. 1
Antibody Details
30C7
The 30C7 monoclonal antibody specifically binds to CD229.1, the Ly-9.1 (Lgp-100a) surface glycoprotein alloantigen found on most thymocytes, peripheral T and B cells, bone marrow lymphoid cells, hematopoietic progenitors, and lymphomas of most strains (e.g., AKR, Balb/c, CBA, SJL). 30C7 mAb does not react with the Ly-9.2 alloantigen expressed on mice derived from the C57/J line (e.g., C57BL, C57BR, C57L, C58).
560201 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
560201 Rev.1
Citations & References
Development References (4)
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Lanier LL, Warner NL, Ledbetter JA, Herzenberg LA. Quantitative immunofluorescent analysis of surface phenotypes of murine B cell lymphomas and plasmacytomas with monoclonal antibodies. J Immunol. 1981; 127(4):1691-1697. (Clone-specific). View Reference
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Ledbetter JA, Goding JW, Tsu TT, Herzenberg LA. A new mouse lymphoid alloantigen (Lgp100) recognized by a monoclonal rat antibody. Immunogenetics. 1979; 8(1):347-360. (Immunogen).
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Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979; 47:63-90. (Biology). View Reference
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Miller BA, Antognetti G, Springer TA. Identification of cell surface antigens present on murine hematopoietic stem cells. J Immunol. 1985; 134(5):3286-3290. (Biology). View Reference
560201 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
