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      PE Rat Anti-Mouse CD2
      Product Details
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      BD Pharmingen™
      LFA-2; LFA-3 receptor; Ly37; Ly-37; Lymphocyte antigen 37; T11
      Mouse (QC Testing)
      Rat SD, also known as Sprague-Dawley (outbred) IgG2b, λ
      Mouse BALB/c Thymocytes
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      12481
      AB_2073810
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      553112 Rev. 12
      Antibody Details
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      RM2-5

      The RM2-5 monoclonal antibody specifically binds to the immunoglobulin superfamily adhesion molecule, CD2 (LFA-2). CD2 is a type I transmembrane glycoprotein that serves as the the major receptor for CD48 in the mouse. CD2 is involved in T-cell activation, immunoregulation, and thymocyte maturation. In the mouse, CD2 is expressed on peripheral T lymphocytes, B lymphocytes, and NK cells, and a subpopulation of intraepithelial T lymphocytes. CD2 is expressed throughout mouse thymic ontogeny, except for distinct subsets of the CD4-CD8- early thymocytes. In the mouse bone marrow, CD2 is expressed on B220+ sIg+ CD43- pre-B cells, but not on CD43+ pro-B cells. The RM2-5 antibody is one of a set of five anti-mouse CD2 monoclonal antibodies that were classified into two groups according to their mutual competition in binding to cell surface CD2 and which block CD2-mediated cell-cell adhesion.

      553112 Rev. 12
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      553112 Rev.12
      Citations & References
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      Development References (16)

      1. Ayroldi E, Migliorati G, Cannarile L, Moraca R, Delfino DV, Riccardi C. CD2 rescues T cells from T-cell receptor/CD3 apoptosis: a role for the Fas/Fas-L system. Blood. 1997; 89(10):3717-3726. (Biology). View Reference
      2. Cibotti R, Punt JA, Dash KS, Sharrow SO, Singer A. Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals. Immunity. 1997; 6(3):245-255. (Clone-specific: Induction). View Reference
      3. Criado G, Feito MJ, Rojo JM. CD4-dependent and -independent association of protein tyrosine kinases to the T cell receptor/CD3 complex of CD4+ mouse T lymphocytes. Eur J Immunol. 1996; 26(6):1228-1234. (Clone-specific: Induction). View Reference
      4. Davis SJ, van der Merwe PA. The structure and ligand interactions of CD2: implications for T-cell function. Immunol Today. 1996; 17(4):177-187. (Biology). View Reference
      5. Hayday A, Theodoridis E, Ramsburg E, Shires J. Intraepithelial lymphocytes: exploring the Third Way in immunology. Nat Immunol. 2001; 2(11):997-1003. (Biology). View Reference
      6. Kato K, Koyanagi M, Okada H, et al. CD48 is a counter-receptor for mouse CD2 and is involved in T cell activation. J Exp Med. 1992; 176(5):1241-1249. (Biology). View Reference
      7. Kuo S, El Guindy A, Panwala CM, Hagan PM, Camerini V. Differential appearance of T cell subsets in the large and small intestine of neonatal mice. Pediatr Res. 2001; 49(4):543-551. (Biology). View Reference
      8. Masten BJ, Yates JL, Pollard Koga AM, Lipscomb MF. Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L. Am J Respir Cell Mol Biol. 1997; 16(3):335-342. (Clone-specific: Blocking). View Reference
      9. Nakamura T, Takahashi K, Fukazawa T, et al. Relative contribution of CD2 and LFA-1 to murine T and natural killer cell functions. J Immunol. 1990; 145(11):3628-3634. (Immunogen: Flow cytometry). View Reference
      10. Papavasiliou F, Misulovin Z, Suh H, Nussenzweig MC. The role of Ig beta in precursor B cell transition and allelic exclusion. Science. 1995; 268(5209):408-411. (Biology). View Reference
      11. Rakasz E, Hagen M, Sandor M, Lynch RG. Gamma delta T cells of the murine vagina: T cell response in vivo in the absence of the expression of CD2 and CD28 molecules. Int Immunol. 1997; 9(1):161-167. (Biology). View Reference
      12. Rodewald HR, Awad K, Moingeon P, et al. Fc gamma RII/III and CD2 expression mark distinct subpopulations of immature CD4-CD8- murine thymocytes: in vivo developmental kinetics and T cell receptor beta chain rearrangement status. J Exp Med. 1993; 177(4):1079-1092. (Biology). View Reference
      13. Teh SJ, Killeen N, Tarakhovsky A, Littman DR, Teh HS. CD2 regulates the positive selection and function of antigen-specific CD4- CD8+ T cells. Blood. 1997; 89(4):1308-1318. (Biology). View Reference
      14. Yagita H, Asakawa J, Tansyo S, Nakamura T, Habu S, Okumura K. Expression and function of CD2 during murine thymocyte ontogeny. Eur J Immunol. 1989; 19(12):2211-2217. (Biology). View Reference
      15. Yagita H, Nakamura T, Asakawa J, et al. CD2 expression in murine B cell lineage. Int Immunol. 1989; 1(1):94-98. (Biology). View Reference
      16. Yagita H, Nakamura T, Karasuyama H, Okumura K. Monoclonal antibodies specific for murine CD2 reveal its presence on B as well as T cells. Proc Natl Acad Sci U S A. 1989; 86(2):645-649. (Immunogen: Blocking, Immunoprecipitation). View Reference
      View All (16) View Less
      553112 Rev. 12

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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