Mouse IgG1 PE

BD™ Mouse IgG1 PE

Name: Mouse IgG1 PE
Brand: BD™
SKU: 340760

Clone X40

(ASR)

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD™

Alternative Name: IgG1, kappa Isotype Control (Anti-KLH)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Keyhole limpet hemocyanin (KLH)

Application: Flow cytometry

Concentration: 25 µg/mL

Vol. Per Test: 20 μL

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: ASR

Preparation And Storage

Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340760 Rev. 1

Antibody Details

X40

The Mouse IgG₁ antibody, clone X40, is derived from the hybridization of Sp2/0-Ag 14 mouse myeloma cells with spleen cells from BALB/c mice immunized with KLH.

The antibodies react specifically with keyhole limpet hemocyanin (KLH), an antigen not expressed on human cells or human cell lines.

340760 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

340760 Rev.1

Citations & References

Development References (2)

Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).

340760 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.