North America
South America
Australia & New Zealand
Europe
Middle East / Africa
Asia / Pacific
×
×
PE Mouse anti-Mouse Ly-108 13G3 RUO

PE Mouse anti-Mouse Ly-108 

Clone  13G3   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name CD352; Slamf6; SLAM family member 6; KAL1; NTB-A; SF2000
  • Concentration 0.2 mg/ml
  • Isotype Mouse IgG2a, κ
  • Reactivity Mouse (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen WT thymocytes
  • Entrez Gene ID 30925 
  • Storage Buffer Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
  • Regulatory Status RUO
Detalhes do produto Recommended Assay Referências

Description

The 13G3 monoclonal antibody specifically binds to Lymphocyte antigen 108, Ly-108. Ly-108 is a member of the signaling lymphocytic activation molecule (SLAM) family of immune receptors. Ly-108 is a type 1 transmembrane glycoprotein adhesion receptor that is expressed by T cells, NKT cells, B cells, NK cells, macrophages, dendritic cells and neutrophils. Ly-108 plays multiple roles in innate and adaptive immunity including costimulation of NK cell cytotoxicity and T cell cytokine responses. Moreover, Ly-108 has been implicated in autoimmunity.

FORMATO
  • Format PE
  • Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
  • Excitation Max 496 nm
  • Emission Max 578 nm

R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.

Other Formats →

Suggested Companion Products

Stain Buffer (FBS) RUO
500 mL Cat No: 554656

Alexa Fluor® 647 Rat Anti-Mouse CD45R RA3-6B2 RUO
0.1 mg Cat No: 557683

V450 Hamster Anti-Mouse CD3e 500A2 RUO
0.1 mg Cat No: 560801

RECURSOS E FERRAMENTAS
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.


  1. Griewank K, C Borowski, Rietdijk S, et al. Homotypic interactions mediated by Slamf1 and Slamf6 receptors control NKT cell lineage development. Immunity. 2007; 27(5):751-762. (Clone-specific: Flow cytometry). View reference

  2. Howie D, Laroux FS, Morra M, et al. Cutting edge: the SLAM family receptor Ly108 controls T cell and neutrophil functions. J Immunol. 2005; 10(174):5931-5935. (Biology: Flow cytometry). View reference

  3. Li W, Sofi MH, Rietdijk S, Wang N, et al. The SLAM-Associated Protein (SAP)/Fyn/PKCθ Pathway is Required for Thymocyte-mediated CD4 T Cell Development. Immunity. 2007; 27(2):763-774. (Biology: Flow cytometry). View reference

  4. Peck SR, Ruley HE. Ly108: a new member of the mouse CD2 family of cell surface proteins. J Immunol. 2000; 52(1-2):63-72. (Biology: Flow cytometry). View reference

561540 Rev. 1
Instruments
Reagents
Applications
Sales & Support
BD BioSciences