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FITC Rat Anti-Mouse CD3 Molecular Complex
FITC Rat Anti-Mouse CD3 Molecular Complex
CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with FITC-conjugated 17A2 (Cat. No. 555274) and PE-conjugated anti-mouse CD19 mAb 1D3 (Cat. No. 557399, left panel). BALB/c thymocytes were also stained with FITC-conjugated 17A2 mAb (Cat. No. 555274, right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with FITC-conjugated 17A2 (Cat. No. 555274) and PE-conjugated anti-mouse CD19 mAb 1D3 (Cat. No. 557399, left panel). BALB/c thymocytes were also stained with FITC-conjugated 17A2 mAb (Cat. No. 555274, right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Product Details
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BD Pharmingen™
CD3; CD3 epsilon; Cd3e; CD3ε; T3e
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
γδ TCR-positive T-T hybridoma D1
Flow cytometry (Routinely Tested)
0.5 mg/ml
12501
AB_395698
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
561798 Rev. 2
Antibody Details
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17A2

The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11 (Cat. No. 557306/553058/550275). This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells.

561798 Rev. 2
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
561798 Rev.2
Citations & References
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Development References (2)

  1. Miescher GC, Schreyer M, MacDonald HR. Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol Lett. 1989; 23(2):113-118. (Immunogen: Cytotoxicity, Functional assay, Immunohistochemistry, Immunoprecipitation, Stimulation). View Reference
  2. Mysliwietz J, Thierfelder S. Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in the marrow recipient. Blood. 1992; 80(10):2661-2667. (Clone-specific: Depletion). View Reference
561798 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.