Fixation/Permeabilization Solution Kit with BD GolgiStop™
- Brand BD Cytofix/Cytoperm™ Plus
- Contents Fixation/Permeabilization solution (125 ml), BD Perm/Wash™ Buffer (100 ml) and BD GolgiStop™ protein transport inhibitor (containing Monensin)
- Regulatory Status RUO
Regulatory Status Legend
The BD Cytofix/Cytoperm Plus Fixation/Permeabilization Kit provides Fixation/Permeabilization solution, BD Perm/Wash™ Buffer, and BD GolgiStop™ protein transport inhibitor containing monensin. The ex vivo addition of BD GolgiStop™ to in vitro- or in vivo-stimulated cells blocks their intracellular transport processes. This results in the accumulation of most cytokine proteins in the Golgi complex and thereby enhances cytokine staining signals. Enough BD GolgiStop reagent is provided for treating up to 1 liter of cell culture at a cell density of up to 2 × 106 cells/ml.
Components Quantity Fixation/Permeabilization solution 125 mL BD Perm/Wash™ Buffer (10x) 100 mL BD GolgiStop protein transport inhibitor containing monensin 0.7 mL