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AmCyan Mouse Anti-Human CD45
Product Details
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BD™
PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5
Human
Mouse IgG1, κ
Flow cytometry
100 μg/mL
5 μL
Phosphate buffered saline with BSA and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

339192 Rev. 1
Antibody Details
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2D1

The CD45 antibody, clone 2D1, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood mononuclear cells (PBMCs).

The CD45 antibody (anti–HLe-1) recognizes members of the T200 family of human leucocyte antigens with molecular mass of 180 to 220 kilodaltons (kDa.)

339192 Rev. 1
Format Details
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AmCyan
The AmCyan™ dye is part of the BD violet family of dyes. This dye is a fluorescent protein with an excitation maximum (Ex Max) of 457-nm and an emission maximum (Em Max) at 489 nm. AmCyan is designed to be excited by the Violet laser (405-nm) and detected using an optical filter centered near 500-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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AmCyan
339192 Rev.1
Citations & References
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Development References (8)

  1. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  2. Clinical and Laboratory Standards Institute. 2005. (Biology).
  3. Hermiston ML, Xu Z, Weiss A. CD45: a critical regulator of signaling thresholds in immune cells. Annu Rev Immunol. 2003; 21:107-137. (Biology). View Reference
  4. Jackson A. Basic phenotyping of lymphocytes: selection and testing of reagents and interpretation of data. Clin Immunol Newslett. 1990; 10:43-55. (Biology).
  5. Loken MR, Brosnan JM, Bach BA, Ault KA. Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry. Cytometry. 1990; 11(4):453-459. (Biology). View Reference
  6. Pellat-Deceunynck C, Bataille R. Normal and malignant human plasma cells: proliferation, differentiation, and expansions in relation to CD45 expression. Blood Cells Mol Dis. 2004; 32:293–301. (Biology).
  7. Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
  8. Terstappen LW, Levin J. Bone marrow cell differential counts obtained by multidimensional flow cytometry. Blood Cells. 1992; 18(2):311-330. (Biology). View Reference
View All (8) View Less
339192 Rev. 1

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Although not required, these products are manufactured in accordance with Good Manufacturing Practices.