UV (High Intensity) Fluorescent Particles, 1.7 - 2.2 µm

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Sphero™ UV (High Intensity) Fluorescent Particles, 1.7 - 2.2 µm

Name: UV (High Intensity) Fluorescent Particles, 1.7 - 2.2 µm
Brand: Sphero™
SKU: 556255

(RUO)

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UV (High Intensity) Fluorescent Particles, 1.7 - 2.2 µm

Excitation and emission spectra of SPHERO fluorescent particles in suspension with the exception of Nile Blue and Sky blue, which were read from a solution of dye in styrene.

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Scientific Resources

Product Details

Description

The vial contains a mixture of 1.7 - 2.2 µm UV Fluorescent Particles that are dyed to have an excitation maximum of approximately 360 nm with a subsequent emission maximum of approximately 415 nm. This particle mixture is useful with flow cytometers equipped with a multi-line UV laser. This particle mixture has compatible excitation and emission spectra with the Hoeschst 33342 and 33258 fluorophores.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

This particle mixture (~1.0% w/v) is useful for routine calibration of flow cytometers. Before use, resuspend the particles by vortexing. Dilution of 3 - 5 drops of particles to 1 ml of sheath fluid will provide an adequate number of particles for flow cytometric analysis.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

556255 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.