Preparation And Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
The CD95, clone DX2, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from C3H/He mice immunized with L cells transfected with CD95 antigen. The CD95 recognizes a 48-kdalton (kDa) antigen (Fas/APO-1) that is a member of the tumor necrosis factor/nerve growth factor (TNF/NGF) superfamily. It is associated with activated cells. Cross linking of CD95 antigen on activated cells can elicit apoptosis.
Development References (11)
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Amasaki Y, Kobayashi S, Takeda T, et al. Up-regulated expression of Fas antigen (CD95) by peripheral naive and memory T cell subsets in patients with systemic lupus erythematosus (SLE): A possible mechanism for lymphopenia. Clin Exptl Immunol. 1995; 99:245-250. (Biology).
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Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
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Cifone MG, De Maria R, Roncaioli P, et al. Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase. J Exp Med. 1994; 180(4):1547-1552. (Biology). View Reference
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McClosky TW, Oyaizu N, Pahwa S. Review: Apoptosis, HIV infection, and the Fas antigen. Clin Immunol Newsletter. 1995; 15:105-113. (Biology).
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Miyawaki T, Uehara T, Nibu R, et al. Differential expression of apoptosisrelated Fas antigen on lymphocyte subpopulations in human peripheral blood. J Immunol. 1992; 149:3753-3758. (Biology).
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Möller P, Koretz K, Leithäuser F, et al. Expression of APO-1 (CD95), a member of the NFG/TNF superfamily, in normal and neoplastic colon epithelium. Int J Cancer. 1994; 57:371-377. (Biology).
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Oehm A, Behrmann I, Falk W, Pawlita M, et al. Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. J Biol Chem. 1992; 140:197-205. (Biology).
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Owen-Schaub L, Angelo L, Radinsky R, Ware C, et al Soluble Fas/APO-1 in tumor cells: A potential regulator of apoptosis? Cancer Letters. 1992; 140:197-205. (Biology).
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Owen-Schaub LB, Yonehara S, Crump III WL, Grimm EA. DNA fragmentation and cell death is selectively triggered in activated human lymphocytes by Fas antigen engagement. Cell Immunol. 1992; 140:197-205. (Biology).
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Robertson MJ, Ritz J. Knapp W, Dörken B, Gilks W, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:1142-3.
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Shima Y, Nishimoto N, Ogata A, Fujii Y, Yoshizaki K, Kishimoto T. Myeloma cells express Fas antigen/APO-1 (CD95) but only some are sensitive to anti-Fas antibody resulting in apoptosis. Blood. 1995; 85:757-764. (Biology).
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
