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FITC BrdU Flow Kit

BD Pharmingen™ FITC BrdU Flow Kit

(RUO)
Product Details
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BD Pharmingen™
Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)
RUO
AB_2617060


Preparation And Storage

Antibody, buffers, and 7-AAD. Store the fluorochrome-conjugated anti-BrdU antibody, BD Cytofix/Cytoperm Buffer, BD Perm/Wash Buffer, BD Cytoperm Permeabilization Buffer Plus, and 7-AAD at 2 to 8°C. Keep the fluorochrome-conjugated anti-BrdU antibody and 7-AAD protected from light. Unused portions of 1X BD Cytofix/Cytoperm Buffer can be stored at 2 to 8°C. BrdU and DNase. Store the BrdU and DNase at –80°C. The BrdU solution has been shown to be stable for up to 4 months at 2 to 8°C, or it can be refrozen. Avoid multiple freeze-thaw cycles. DNase stock solution (1 mg/mL) may be refrozen once before it loses activity

559619 Rev. 1
Citations & References
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Development References (27)

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  5. Gonchoroff NJ, Katzmann JA, Currie RM, et al. S-phase detection with an antibody to bromodeoxyuridine. Role of DNase pretreatment. J Immunol Meth. 1986; 93:97-101. (Biology).
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  8. Holm M, Thomsen M, Hoyer M, Hokland P. Optimization of a flow cytometric method for the simultaneous measurement of cell surface antigen, DNA content, and in vitro BrdUrd incorporation into normal and malignant hematopoietic cells. Cytometry. 1998; 32(1):28-36. (Biology). View Reference
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  11. Lakhanpal S, Gonchoroff NJ, Katzmann JA, Handwerger BS. A flow cytofluorometric double staining technique for simultaneous determination of human mononuclear cell surface phenotype and cell cycle phase. J Immunol Meth. 1987; 96:35-40. (Biology).
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559619 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.