The BD Biosciences Webinar Series is an ongoing, informative program in which senior scientists, professionals in the community, and clinicians present online seminars covering a broad range of topics.

A 10-color, Single-tube Panel for Detection of Minimal Residual Disease in Multiple Myeloma

Date: Wednesday, August 12, 2015
Time: 12 PM EDT / 9 AM PDT / 5 PM BST / 6 PM CEST
Mikhail Roshal, MD, PhD
Memorial Sloan Kettering Cancer Center

Memorial Sloan Kettering Cancer Center

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A growing body of evidence supports the utility of multicolor flow cytometry for detecting minimal residual disease (MRD) in multiple myeloma patients. However, with the National Cancer Institute’s recommendation of at least a 10-marker panel, acquiring a minimum of 3–5 million cells per tube burdens laboratory workflow at centers using 8-color flow cytometry. Many centers are confronted with the prospect of providing a 2-tube, 10-marker panel for myeloma MRD. This approach places significant demands on instrument use, data storage, and analysis time.

In this webcast, Dr. Mikhail Roshal and Qi Gao will discuss a novel single-tube, 10-color panel that they designed and validated for myeloma MRD. This panel incorporates the recommended National Cancer Institute markers and was compared with their single-tube, 8-color plasma cell MRD panel. The novel panel offers the prospect of improving flow cytometry resource utilization and reducing the inferential reasoning required by multiple-tube panels.


Mikhail Roshal, MD, PhD, is the Director of Development in Flow Cytometry and attending physician in Hematopathology at the Memorial Sloan Kettering Cancer Center. He received his MD and PhD from University of Rochester and completed his residency and fellowship at the University of Washington. One area of research for Dr. Roshal is flow cytometric identification of neoplastic hematologic populations.

Qi Gao, MLS(ASCP) CM, is a clinical laboratory scientist at the Cell Marker Lab at the Memorial Sloan Kettering Cancer Center, where she has validated 10-color panel designs. She received a BS in clinical laboratory science from Stony Brook University.

Support for this program was provided by BD. Development of content was solely the responsibility of the speakers. Views and opinions expressed are their own.

By attending the webinar you may receive continuing education units from both P.A.C.E.* and CE Broker** to fulfill your yearly requirements for maintaining your professional license.

* P.A.C.E. is a registered trademark of the American Society for Clinical Laboratory Science (ASCLS). BD Biosciences is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program.
** BD Biosciences is a Florida Board of Clinical Laboratory Personnel approved CEU provider. CE Broker is the official tracking system used by the Florida Department of Health.

Enabling a Deeper Understanding of What is on, in, and Made by the Cell

Date: September 15, 2015
Time: 12 PM EDT / 9 AM PDT / 5 PM BST / 6 PM CEST
Robert Balderas
Vice President Biological Sciences, BD Biosciences

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Multiple aspects of a cell need to be studied for a deeper understanding of cell biology. Changes in cellular processes and protein expression can occur upon specific treatments. Apoptosis inducers, for example, trigger a cascade of events, including cleavage of caspases, which will eventually lead to cell death. Treatment with proinflammatory cytokines activates cell signaling pathways resulting in phosphorylation of key signal transducers and increased secretion of specific cytokines. All of these cellular responses can be measured at the single-cell level by quantitative and multiparametric flow cytometry assays. During this webinar, we will discuss how combining the BD Accuri™ C6 personal flow cytometer with BD Biosciences reagents provides a unique research solution to facilitate cell biology research. The specific topics that will be discussed are:

  • Methods to optimize and multiplex the analysis of viability, apoptosis, and cell proliferation
  • Best practices for quick and robust analysis of cell signaling and protein phosphorylation
  • Use of BD™ Cytometric Bead Array (CBA) system for sensitive and quantitative analysis of multiple cytokines


Bob Balderas 091515 Webinar

Robert Balderas, has more than 35 years of leadership experience in both academia and the biotechnology industry. As a corporate scientist, site manager, and marketing professional in the field of biotechnology, he has contributed to the growth of both Pharmingen and BD Biosciences.

Robert has championed multiple key successful product launches during his tenure at Pharmingen and BD Biosciences and is recognized for his success in growing sales and profits. His experience in academic medicine accompanied by his strategic approaches as the VP of Research and Development are reflected in increased efficiencies within the clinical and research product development programs, where he oversees the development of more than 4,000 reagents.

Robert Balderas’ track record of business process improvements is based on expertise he obtained while working at The Scripps Research Institute in the labs of Frank Dixon and Argyrios Theofilopoulos. There, for 18 years, he focused his research in the field of autoimmunity. He is known for his ability to motivate his technical staff, diagnosing impediments in product development programs, building his efforts and focusing on both product execution and the career development of his staff, as well as integrating functional programs across other business operations. Today, as VP of Market Development, Robert is focused on driving market adoption of new products and technologies into the life sciences community and is a core domain leader for the flow cytometry platform.

In addition to his scientific experiences, Robert also received an MBA from the University of San Diego (UCSD). Additionally, Robert serves on the editorial board of Clinical Proteomics, has been involved with several business acquisitions, and led two industrial/academic initiatives with Canadian Network for Visual Analytics (CANVAC) and the National Cancer Institute (NCI). He is a member of the UCSD Industrial Liaison Program, the Deans Council for the UCSD School of Biological Sciences, a corporate member of the UCSD Jacobs School of Engineering, a board member of the Human Leukocyte Differentiation Antigen (HLDA), an SAB member of Inflamex (Paris, France), an SAB Member UC Davis Photonics and leads our BD Biosciences Technology Leadership Development Program (TLDP) recruiting team. He has shared in the publishing of over 80 scientific journal articles, and is a recipient of the BD Wesley J. Howe Award for “Sensing, Sourcing and Seeking New Technologies.”

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