Skip to main content Skip to navigation
BV711 Rat Anti-Mouse CD314
BV711 Rat Anti-Mouse CD314
Two-color flow cytometric analysis of CD314 (NKG2D) expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse CD49b antibody (Cat. No. 560628) and either BD Horizon™ BV711 Rat IgG1 Isotype Control (Cat. No. 563283, Left Panel) or BD Horizon™ BV711 Rat Anti-Mouse CD314 antibody (Cat. No. 563694, Right Panel). Two-color flow cytometric dot plots showing CD314 or Ig Isotype control staining versus CD49b expression were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry was performed on a BD™ LSR II Flow Cytometer System.
Two-color flow cytometric analysis of CD314 (NKG2D) expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse CD49b antibody (Cat. No. 560628) and either BD Horizon™ BV711 Rat IgG1 Isotype Control (Cat. No. 563283, Left Panel) or BD Horizon™ BV711 Rat Anti-Mouse CD314 antibody (Cat. No. 563694, Right Panel). Two-color flow cytometric dot plots showing CD314 or Ig Isotype control staining versus CD49b expression were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry was performed on a BD™ LSR II Flow Cytometer System.
Product Details
Down Arrow Up Arrow


BD Horizon™
Klrk1; NKG2D; NKG2-D; NK cell receptor D; Natural killer cell group 2D
Mouse (QC Testing)
Rat IgG1
Purified Mouse NKG2D protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
27007
AB_2722498
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV711 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV711 were removed.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. BD Horizon Brilliant Violet 711 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. Cy is a trademark of GE Healthcare.
  10. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  11. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
563694 Rev. 2
Antibody Details
Down Arrow Up Arrow
CX5

The CX5 monoclonal antibody specifically binds to NKG2D, also known as CD314. NKG2D is a lectin-like receptor that is detected on resting and IL-2-activated NK cells, activated CD8-positive T lymphocytes, and LPS-activated macrophages, but not on resting T cells or unstimulated macrophages.  NKG2D has little homology to the other members of the NKG2 family, NKG2A, C, and E, and does not form heterodimers with CD94.  On NK cells, NKG2D is an activating receptor that associates with DAP10, an adapter protein that stimulates the PI3 kinase pathway.  An isoform of mouse NKG2D can also associate with the signaling adapter protein DAP12, also known as KARAP (Killer cell-Associated Receptor-Associated Polypeptide), that activates the Syk and ZAP70 tyrosine kinases.  On cytotoxic T cells, NKG2D is a co-stimulatory receptor that associates with DAP10.  The ligands for NKG2D include the minor histocompatibility antigen H60, MULT1 (Murine UL16-binding protein-Like Transcript 1), and the five retinoic acid-inducible proteins Rae-1α, β, γ, δ and ε.  Interactions of NKG2D with its ligands are involved in the regulation of innate and immune cytotoxic responses to tumor and pathogen-infected cells and in diabetes progression in the NOD mouse.  The CX5 mAb blocks the binding of NKG2D to its ligands.

The antibody was conjugated to BD Horizon BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm.  BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor® 700-like dyes (eg, 712/20-nm filter).  Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors.  However, the spillover can be corrected through compensation as with any other dye combination.

563694 Rev. 2
Format Details
Down Arrow Up Arrow
BV711
The BD Horizon Brilliant Violet™ 711 (BV711) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 713-nm. BV711, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 710-nm (e.g., a 712/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
BV711
Violet 405 nm
407 nm
713 nm
563694 Rev.2
Citations & References
Down Arrow Up Arrow

Development References (8)

  1. Carayannopoulos LN, Naidenko OV, Fremont DH, Yokoyama WM. Murine UL16–binding protein–like transcript 1: A newly described transcript encoding a high–affinity ligand for murine NKG2D . J Immunol. 2002; 169(8):4079-4083. (Biology). View Reference
  2. Cerwenka A, Bakker AB, McClanahan T, et al. Retinoic acid early inducible genes define a ligand family for the activating NKG2D receptor in mice. Immunity. 2000; 12(6):721-727. (Biology). View Reference
  3. Diefenbach A, Jamieson AM, Liu SD, Shastri N, Raulet DH. Ligands for the murine NKG2D receptor: expression by tumor cells and activation of NK cells and macrophages. Nat Immunol. 2000; 1(2):119-126. (Biology). View Reference
  4. Diefenbach A, Tomasello E, Lucas M, et al. Selective associations with signaling proteins determine stimulatory versus costimulatory activity of NKG2D. Nat Immunol. 2002; 3(12):1142-1149. (Biology). View Reference
  5. Gilfillan S, Ho EL, Cella M, Yokoyama WM, Colonna M. NKG2D recruits two distinct adapters to trigger NK cell activation and costimulation . Nat Immunol. 2002; 3(12):1150-1155. (Biology). View Reference
  6. Ogasawara K, Hamerman JA, Ehrlich LR, et al. NKG2D blockade prevents autoimmune diabetes in NOD mice. Immunity. 2004; 20(6):757-767. (Clone-specific: Blocking). View Reference
  7. Ogasawara K, Hamerman JA, Hsin H, et al. Impairment of NK cell function by NKG2D modulation in NOD mice. Immunity. 2003; 18(1):41-51. (Immunogen: Blocking). View Reference
  8. Yokoyama WM. Natural killer cell receptors. Curr Opin Immunol. 1998; 10(3):298-305. (Biology). View Reference
View All (8) View Less
563694 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.