BV650 Mouse Anti-Human CD166 Clone 3A6 (RUO)

Brand: BD OptiBuild™

BD OptiBuild custom reagents make BD Horizon Brilliant™ dyes readily available across a wide selection of cell surface antibodies. Learn more about what makes BD OptiBuild reagents unique Learn more at bdbiosciences.com/go/optibuild
Alternative Name: ALCAM; Activated leucocyte cell adhesion molecule; MEMD
Concentration: 0.2 mg/ml
Isotype: Mouse IgG₁, κ
Reactivity: Human (Tested in Development)
Application: Flow cytometry (Qualified)
Workshop No.: VI A109
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO

Sizes: 50 µg
Catalog No.: 742374
List Price: $495.00
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Unit: EA (1 Each)
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Description

The 3A6 monoclonal antibody specifically binds to the activated leukocyte cell adhesion molecule (ALCAM), a 100-105 kDa transmembrane glycoprotein, also known as the CD6 ligand, CD166. It is composed of an immunoglobulin-like extracellular domain, a transmembrane region and a short cytoplasmic tail. ALCAM belongs to the Ig superfamily of proteins and is expressed on neurons, activated T cells, activated monocytes, epithelial cells and fibroblasts. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T-cell development.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Format

Format: BV650
Excitation Source: Violet 405 nm
Excitation Max: 407 nm
Emission Max: 650 nm

BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.

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Suggested Companion Products

Cat No.	Description	Size
554656	Stain Buffer (FBS) RUO	500 mL
554657	Stain Buffer (BSA) RUO	500 mL
563794	Brilliant Stain Buffer RUO	100 Tests
555899	Lysing Buffer RUO	100 mL
349202	Lysing Solution 10X Concentrate IVD	100
564219	Human BD Fc Block™ RUO	50 mg
563231	BV650 Mouse IgG1, k Isotype Control RUO	50 µg

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Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV650 under optimal conditions that minimize unconjugated dye and antibody.

Product Notices

This antibody was developed for use in flow cytometry.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Researchers should determine the optimal concentration of this reagent for their individual applications.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
BD Horizon Brilliant Violet 650 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
Alexa Fluor® is a registered trademark of Life Technologies Corporation.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Aruffo A, Bowen MA, Patel DD, et al. CD6-ligand interactions: a paradigm for SRCR domain function?. Immunol Today. 1997; 18(10):498-504. View reference
Bowen MA, Bajorath J, Aruffo A. CD166 (ALCAM) Workshop Panel Report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997; :459-461.
Bowen MA, Patel DD, Li X, et al. Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand. J Exp Med. 1995; 181(6):2213-2220. View reference
Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997; .
Patel DD, Wee SF, Whichard LP, et al. Identification and characterization of a 100-kD ligand for CD6 on human thymic epithelial cells. J Exp Med. 1995; 181(4):1563-1568. View reference