Skip to main content Skip to navigation
Alexa Fluor® 647 Mouse Anti-Human Fcµ Receptor

BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human Fcµ Receptor

Clone HM14-1 (also known as HM14)

(RUO)
Alexa Fluor® 647 Mouse Anti-Human Fcµ Receptor
Multicolor flow cytometric analysis of Fcμ Receptor expression on human peripheral blood mononuclear cells. Peripheral blood mononuclear cells were stained with Alexa Fluor® 647 Mouse Anti-Human Fcμ Receptor (Cat. No. 563535), FITC Mouse Anti-Human CD3 (Cat. No. 555332/561806/561807), and BD Horizon™ BV421 Mouse Anti-Human CD11b (Cat. No. 562632) antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of CD3 (Left Panel) or CD11b (Right Panel) versus Fcμ Receptor for gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of Fcμ Receptor expression on human peripheral blood mononuclear cells. Peripheral blood mononuclear cells were stained with Alexa Fluor® 647 Mouse Anti-Human Fcμ Receptor (Cat. No. 563535), FITC Mouse Anti-Human CD3 (Cat. No. 555332/561806/561807), and BD Horizon™ BV421 Mouse Anti-Human CD11b (Cat. No. 562632) antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of CD3 (Left Panel) or CD11b (Right Panel) versus Fcμ Receptor for gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
Down Arrow Up Arrow


BD Pharmingen™
FcμR; FcmR; Fc mu receptor; IgM Fc receptor; FAIM3; TOSO
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human IgM Fc Receptor Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µl
AB_2738265
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
563535 Rev. 1
Antibody Details
Down Arrow Up Arrow
HM14-1

The HM14-1 monoclonal antibody specifically binds to the FAIM3-encoded high affinity receptor for IgM (also known as, Fcµ Receptor, FcμR, Fc mu receptor, Immunoglobulin mu Fc receptor, FcmR, IgM Fc receptor). The FcμR is a type 1 transmembrane glycoprotein that contains an extracellular Ig-like domain with sequence similarities to Fcα/μR and pIgR. It is likewise known as Fas apoptotic inhibitory molecule 3, TOSO or Regulator of Fas-induced apoptosis Toso based on its capacity to counter apoptosis mediated by Fas/CD95 (or TNF) in some experimental model systems. FcμRs are expressed by B cells, CD4+ T cells, CD8+ T cells, and NK cells and are highly expressed on Chronic Lymphocytic Leukemia cells. FcμRs may play roles in cellular activation, antigen presentation and IgM-dependent cell-mediated cytotoxicity. The relatively long cytoplasmic tail of FcμR contains several conserved tyrosine and serine residues. These get phosphorylated after FcμR ligation with IgM immune complexes and may initiate signaling responses. Lymphocyte FcμRs can mediate the internalization of IgM-bound antigens including microbial and viral antigens. This in turn may cause cellular activation due to subsequent antigen processing and exposure of activating ligands to intracellular receptors including Toll-like receptors. The HM7-10 antibody, a related FcμR-specific antibody, reportedly recognizes an epitope near the IgM ligand binding site of the FcμR whereas the HM14-1 antibody does not.

563535 Rev. 1
Format Details
Down Arrow Up Arrow
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
563535 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (5)

  1. Hitoshi Y, Lorens J, Kitada SI, et al. Toso, a cell surface, specific regulator of Fas-induced apoptosis in T cells. Immunity. 1998; 8(4):461-471. (Biology). View Reference
  2. Kubagawa H, Oka S, Kubagawa Y, et al. Identity of the elusive IgM Fc receptor (FcmuR) in humans.. J Exp Med. 2009; 206(12):2779-2793. (Immunogen: Flow cytometry, Immunoprecipitation, Western blot). View Reference
  3. Shima H, Takatsu H, Fukuda S, et al. Identification of TOSO/FAIM3 as an Fc receptor for IgM.. Int Immunol. 2010; 22(3):149-156. (Biology). View Reference
  4. Vire B, David A, Wiestner A. TOSO, the Fcμ receptor, is highly expressed on chronic lymphocytic leukemia B cells, internalizes upon IgM binding, shuttles to the lysosome, and is downregulated in response to TLR activation. J Immunol. 2011; 187(8):4040-4050. (Biology). View Reference
  5. Wines BD, Trist HM, Farrugia W, et al. A conserved host and pathogen recognition site on immunoglobulins: structural and functional aspects. Adv Exp Med Biol. 2012; 946:87-112. (Biology). View Reference
View All (5) View Less
563535 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.