Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
BM28 is a phosphoprotein that migrates at 125kDa in SDS PAGE with a hyperphosphorylated form migrating as a slightly slower band. The ratio of the phosphorylation states alters with the phases of cell cycle — in M, the "fast" phosphorylated BM28 is the major form found, while in G1 phase, the "slow", hyperphosphorylated BM28 predominates. The cellular localization of BM28 is also cell cycle dependent. In G1, most of the BM28 is chromatin bound, whereas, by M phase, the BM28 is still nuclear, but not associated with the DNA. BM28 is necessary for both entry into S phase and cell division as determined by microinjection inhibition experiments. Microinjection of a BM28 antibody into synchronised cells in G1 phase inhibits DNA replication. When injected during S phase or later, no effect on DNA replication is seen, but cell division is inhibited.
Development References (5)
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Cook JG, Park CH, Burke TW, et al. Analysis of Cdc6 function in the assembly of mammalian prereplication complexes. Proc Natl Acad Sci U S A. 2002; 99(3):1347-1352. (Clone-specific: Western blot). View Reference
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Ishimi Y, Ichinose S, Omori A, Sato K, Kimura H. Binding of human minichromosome maintenance proteins with histone H3. J Biol Chem. 1996; 271(39):24115-24122. (Biology). View Reference
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Shreeram S, Sparks A, Lane DP, Blow JJ. Cell type-specific responses of human cells to inhibition of replication licensing. Oncogene. 2002; 21(43):6624-6632. (Clone-specific: Western blot). View Reference
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Todorov IT, Attaran A, Kearsey SE. BM28, a human member of the MCM2-3-5 family, is displaced from chromatin during DNA replication. J Cell Biol. 1995; 129(6):1433-1445. (Biology). View Reference
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Todorov IT, Pepperkok R, Philipova RN, Kearsey SE, Ansorge W, Werner D. A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division. J Cell Sci. 1994; 107(1):253-265. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
