Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/resources/cellbiology/index.jsp
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
The full-length TrkB gene has been reported to encode for a 145 kDa glycosylated transmembrane tyrosine kinase and neurotrophin receptor. The same gene also has been reported to encode for a 95 kDa glycoprotein that is identical to gp145 [TrkB] at the extracellular domain and transmembrane portion but lacks the intracellular portion. TrkB has been observable in a range of 116-145 kD and 70-95 kD due to various TrkB maturation states, subcellular localizations, and glycosylation states. TrkB belongs to a family of tyrosine kinases that include the TrkA proto-oncogene and TrkC. All have an extracellular ligand-binding domain, a transmembrane region, and intracellular kinase and autophosphorylation domains. TrkB binds the neurotrophins NT3 and NT4/5, as well as brain-derived neurotrophic factor (BDNF), a peptide that helps motor neuron survival and repair. The TrkB tyrosine kinase is activated upon binding to BDNF resulting in autophosphorylation of residues Y670, Y674 and Y675 and the subsequent association of several intracellular proteins like PLCγ, Shc, and PI3-Kinase. TrkB is widely expressed in cells of neuroepithelium and neural crest origin. Some of these include motor neurons, dopamine-producing neurons, and neurons which release γ-aminobutyric acid in the substantia nigra, neocortex, and hippocampus. The two TrkB gene products are differentially expressed in regions of the adult brain.
Development References (7)
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Armanini MP, McMahon SB, Sutherland J, Shelton DL, Phillips HS. Truncated and catalytic isoforms of trkB are co-expressed in neurons of rat and mouse CNS.. Eur J Neurosci. 1995; 7(6):1403-1409. (Biology).
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Du J, Feng L, Yang F, Lu B. Activity- and Ca(2+)-dependent modulation of surface expression of brain-derived neurotrophic factor receptors in hippocampal neurons. J Cell Biol. 2000; 150(6):1423-1434. (Biology: Western blot). View Reference
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Ebendal T. Function and evolution in the NGF family and its receptors. J Neurosci Res. 1992; 32(4):461-470. (Biology). View Reference
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Jovanovic JN, Czernik AJ, Fienberg AA, Greengard P, Sihra TS. Synapsins as mediators of BDNF-enhanced neurotransmitter release. Nat Neurosci. 1992; 3(4):323-329. (Biology: Western blot). View Reference
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Klein R, Conway D, Parada LF, Barbacid M. trkB tyrosine protein kinase gene codes for a second neurogenic receptor that lacks the catalytic kinase domain. Cell. 1990; 61(4):647-656. (Biology). View Reference
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Kryll D, Yacoubian T, Haapasalo A, Castren E, Lo D, Barker P. Subcellular localization of full-length and truncated trk receptor isoforms in polarized neurons and epithelial cells.. J Neurosci. 1999; 19(14):5823-5833. (Biology).
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Muller D, Djebbara-Hannas Z, Jourdain P. Brain-derived neurotrophic factor restores long-term potentiation in polysialic acid-neural cell adhesion molecule-deficient hippocampus. Proc Natl Acad Sci U S A. 2000; 97(8):4315-4320. (Biology: Western blot). View Reference
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