Multicolor flow cytometric analysis of mouse CD370 (Clec9A) expression in spleen cells. BALB/c splenocytes were simultaneously stained with Alexa Fluor® 700 Rat Anti-Mouse CD4 (Cat. No. 557956), APC Rat Anti-Mouse CD8 (Cat. No. 553035), FITC Hamster Anti-Mouse CD11c (Cat. No. 553801) antibodies and either PE Rat IgG2a κ Isotype Control (Cat. No. 553930; Left Plot) or PE Rat Anti-Mouse CD370 (Clec9A) antibody (Cat. No. 562734; RIght Plot). Two color flow cytometric dot blots showing the correlated expression pattern of CD11c and Clec9A (or Ig Isotype control staining) were generated from CD8+ CD4- gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
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Multicolor flow cytometric analysis of mouse CD370 (Clec9A) expression in spleen cells. BALB/c splenocytes were simultaneously stained with Alexa Fluor® 700 Rat Anti-Mouse CD4 (Cat. No. 557956), APC Rat Anti-Mouse CD8 (Cat. No. 553035), FITC Hamster Anti-Mouse CD11c (Cat. No. 553801) antibodies and either PE Rat IgG2a κ Isotype Control (Cat. No. 553930; Left Plot) or PE Rat Anti-Mouse CD370 (Clec9A) antibody (Cat. No. 562734; RIght Plot). Two color flow cytometric dot blots showing the correlated expression pattern of CD11c and Clec9A (or Ig Isotype control staining) were generated from CD8+ CD4- gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
CD370; CLC9A; Clec9a; DNGR-1; C-type lectin domain family 9 member A
Mouse (QC Testing)
Rat WI, also known as Wistar (outbred) IgG2a, κ
Mouse Clec9A Peptide
Flow cytometry (Routinely Tested)
0.2 mg/ml
232414
AB_2737755
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
PE Rat IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
553930
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APC Rat Anti-Mouse CD8a RUO
Size
0.1 mg
Cat No.
553035
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FITC Hamster Anti-Mouse CD11c RUO
Size
0.5 mg
Cat No.
553801
Alexa Fluor® 700 Rat Anti-Mouse CD4 RUO
Size
0.1 mg
Cat No.
557956
562734 Rev. 2
Antibody Details
10B4
The 10B4 monoclonal antibody specifically binds to mouse Clec9A. Mouse Clec9A (C-type lectin domain family member 9A) is also known as DNGR1 (Dendritic cell natural killer lectin group receptor 1). It is a type II membrane protein with a single extracellular C-type lectin domain. Clec9A is a dendritic cell subtype-restricted C-type lectin-like receptor. Clec9A is selectively expressed on plasmacytoid dendritic cells and CD8+ myeloid dendritic cells. Clec9A reportedly serves as a receptor for necrotic cells. It can mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
562734 Rev. 2
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
562734 Rev.2
Citations & References
Development References (3)
-
Caminschi L, Proietto AL, Ahmet F, et al. The dendritic cell subtype restricted C-type lectin Clec9A is a target for vaccine enhancement. Blood. 2008; 112(8):3264-3273. (Immunogen). View Reference
-
Huysamen C, Willment JA, Dennehy KM, Brown GD. CLEC9A is a novel activation C-type lectin-like receptor expressed on BDCA3+ dendritic cells and a subset of monocytes. J Biol Chem. 2008; 283(24):16693-16701. (Biology). View Reference
-
Sancho D, Joffre OP, Keller AM, et al. Identification of a dendritic cell receptor that couples sensing of necrosis to immunity. Nature. 2009; 458(7240):899-903. (Biology). View Reference
562734 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
