Purified NA/LE Hamster Anti-Mouse/Rat CD81

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BD Pharmingen™ Purified NA/LE Hamster Anti-Mouse/Rat CD81

Name: Purified NA/LE Hamster Anti-Mouse/Rat CD81
Brand: BD Pharmingen™
SKU: 562240

Clone Eat2

(RUO)

Product Details

Brand: BD Pharmingen™

Alternative Name: TAPA-1; Tspan28; Tapa1; Tapa-1; Target of the antiproliferative antibody 1

Reactivity: Mouse, Rat (QC Testing)

Isotype: Armenian Hamster IgG, κ

Immunogen: CD81+ mouse B lymphoma 38C13

Application: Flow cytometry (Routinely Tested)

Concentration: 1.0 mg/ml

RRID: AB_11154574

Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

562240 Rev. 1

Antibody Details

Eat2

The Eat2 monoclonal antibody specifically binds to CD81, a 26-kDa nonglycosylated member of the transmembrane 4 integral membrane protein superfamily, expressed by many types of cells. For example, CD81 participates with CD19 and CD21 in the signal transduction complex associated with the B-cell receptor on human B lymphocytes and with the CD4 and CD8 co-receptors on human thymocytes and T lymphocytes. In mouse fetal thymic organ culture, interactions of immature thymocytes with CD81 expressed by thymic stromal cells are required to induce development of T cells with αβ T-cell receptors. Furthermore, CD81 has been shown to play a role in the regulation of rat mastcell degranulation. Despite its important roles in the immune response and wide tissue distribution, CD81-deficient mice are born without obvious developmental abnormalities. However, these mice have abnormal immune responses, and impaired fertility. Eat2 mAb cross-reacts with the rat CD81 antigen.

562240 Rev. 1

Format Details

NA/LE

NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

Format: NA/LE

562240 Rev.1

Citations & References

Development References (10)

Boismenu R, Rhein M, Fischer WH, Havran WL. A role for CD81 in early T cell development. Science. 1996; 271(5246):198-200. (Biology). View Reference
Deng J, Yeung VP, Tsitoura D, DeKruyff RH, Umetsu DT, Levy S. Allergen-induced airway hyperreactivity is diminished in CD81-deficient mice. J Immunol. 2000; 165(9):5054-5061. (Biology). View Reference
Fleming TJ, Donnadieu E, Song CH, Laethem FV, Galli SJ, Kinet JP. Negative regulation of Fc epsilon RI-mediated degranulation by CD81. J Exp Med. 1997; 186(8):1307-1314. (Biology). View Reference
Levy S, Todd SC, Maecker HT. CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system. Annu Rev Immunol. 1998; 16:89-109. (Biology). View Reference
Maecker HT, Do MS, Levy S. Maecker HT, Do MS, Levy S. Proc Natl Acad Sci U S A. 1998; 95(5):2458-2462. (Biology). View Reference
Maecker HT, Levy S. Normal lymphocyte development but delayed humoral immune response in CD81-null mice. J Exp Med. 1997; 185(8):1505-1510. (Biology). View Reference
Maecker HT, Todd SC, Kim EC, Levy S. Differential expression of murine CD81 highlighted by new anti-mouse CD81 monoclonal antibodies. Hybridoma. 2000; 19(1):15-22. (Immunogen). View Reference
Miyazaki T, Müller U, Campbell KS. Normal development but differentially altered proliferative responses of lymphocytes in mice lacking CD81. EMBO J. 1997; 16(14):4217-4225. (Biology). View Reference
Tedder TF, Zhou LJ, Engel P. The CD19/CD21 signal transduction complex of B lymphocytes. Immunol Today. 1994; 15(9):437-442. (Biology). View Reference
Tsitsikov EN, Gutierrez-Ramos JC, Geha RS. Impaired CD19 expression and signaling, enhanced antibody response to type II T independent antigen and reduction of B-1 cells in CD81-deficient mice. Proc Natl Acad Sci U S A. 1997; 94(20):10844-10849. (Biology). View Reference

View All (10)

562240 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.