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      FITC Mouse Anti-Human CD98
      FITC Mouse Anti-Human CD98
      Flow cytometric analysis of CD98 expression on human peripheral blood lymphocytes. Whole blood was stained with either FITC Mouse Anti-Human CD98 (Cat. No. 556076; solid line histogram) or FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system
      FITC Mouse Anti-Human CD98
      Flow cytometric analysis of CD98 expression on human peripheral blood lymphocytes. Whole blood was stained with either FITC Mouse Anti-Human CD98 (Cat. No. 556076; solid line histogram) or FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system
      Product Details
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      BD Pharmingen™
      SLC3A2; CD98HC; 4F2; 4F2hc; MDU1; NACAE; 4T2HC
      Human (QC Testing)
      Mouse BALB/c IgG1, κ
      Human T-leukemic and Thymic Cell Lines
      Flow cytometry (Routinely Tested)
      20 µl
      V T020; BP420
      AB_396343
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      556076 Rev. 7
      Antibody Details
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      UM7F8

      CD98 is a disulfide-linked heterodimer composed of a ~80-85 kDa, type II membrane glycoprotein heavy chain, (CD98hc), and ~40-45 kDa light chain. The UM7F8 monoclonal antibody specifically recognizes CD98hc, which is also known as 4F2 heavy chain antigen (4F2hc). CD98 is broadly expressed on hematopoietic cells, including peripheral blood lymphocytes, monocytes and granulocytes (low), as well as non-hematopoietic cells, eg, intestinal epithelial cells. CD98 expression is upregulated on activated and proliferating cells. CD98hc is encoded by the SLC3A2 [solute carrier family 3 (amino acid transporter heavy chain), member 2] gene. CD98 reportedly functions in transmembrane amino acid transport and in the regulation of integrin signaling which are involved in the regulation of cellular activation, proliferation, and survival. The UM7F8 antibody is reportedly a functional antibody that can costimulate T cell proliferative responses.

      556076 Rev. 7
      Format Details
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      556076 Rev.7
      Citations & References
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      Development References (4)

      1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
      2. Freidman AW, Diaz LA, Jr., Moore S, Schaller J, Fox DA. The human 4F2 antigen: evidence for cryptic and noncryptic epitopes and for a role of 4F2 in human T lymphocyte activation. Cell Immunol. 1994; 154(1):253-263. (Immunogen). View Reference
      3. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
      4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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      556076 Rev. 7

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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