Immunohistochemical staining of human CD20 expressed by cells within human lymphoma tissue. Following antigen retrieval with BD Pharmingen™ Retrievagen A (pH 6.0) (Cat. No. 550524) solution, the sections from a formalin-fixed, paraffin-embedded human lymphoma were stained overnight with either Purified Mouse IgG2a, κ Isotype Control (Cat. No. 555571; Left Figure) or Purified Mouse Anti-Human CD20 antibody (Cat. No. 555677; Right Figure). A three-step staining procedure that employed Biotin Goat Anti-Mouse Ig (Multiple Adsorption) [Cat. No. 550337], Streptavidin HRP (Cat No. 550946), and DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Counter staining was with hematoxylin. Original magnification 20X.
Immunohistochemical staining of human CD20 expressed by cells within human lymphoma tissue. Following antigen retrieval with BD Pharmingen™ Retrievagen A (pH 6.0) (Cat. No. 550524) solution, the sections from a formalin-fixed, paraffin-embedded human lymphoma were stained overnight with either Purified Mouse IgG2a, κ Isotype Control (Cat. No. 555571; Left Figure) or Purified Mouse Anti-Human CD20 antibody (Cat. No. 555677; Right Figure). A three-step staining procedure that employed Biotin Goat Anti-Mouse Ig (Multiple Adsorption) [Cat. No. 550337], Streptavidin HRP (Cat No. 550946), and DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Counter staining was with hematoxylin. Original magnification 20X.
Product Details
BD Pharmingen™
MS4A1; membrane-spanning 4-domains subfamily A member 1; B1; Bp35; LEU-16
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human B lymphoma cell line
Intracellular staining (flow cytometry) (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
0.5 mg/ml
V cB010
AB_396030
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Companion Products
FITC Goat Anti-Mouse IgG/IgM RUO
Size
0.5 mg
Cat No.
555988
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Purified Mouse IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
555571
555677 Rev. 6
Antibody Details
H1
The H1 (FB1) antibody specificially binds to a cytoplasmic domain of CD20. CD20 is a 33-37-kDa four transmembrane phosphoprotein that is expressed by B lymphocytes from the pre-B stage and most malignant B cells and is lost during plasma cell differentiation. Low level CD20 expression is observed on a subset of normal circulating T lymphocytes, and CD20-positive T-cell lymphomas have been reported. The CD20 molecule is associated with membrane lipid raft domains, acts as a channel for calcium ions, and is involved in the regulation of B cell activation and survival. The cytoplasmic domain regions are serine and threonine rich and contain multiple phosphorylation consensus sequences.
555677 Rev. 6
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555677 Rev.6
Citations & References
Development References (5)
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Hultin LE, Hausner MA, Hultin PM, Giorgi JV. CD20 (pan-B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytes. Cytometry. 1993; 14(2):193-204. (Biology). View Reference
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Nozawa Y, Abe M, Ohno H, Fukuhara S, Wakasa H. Production of two monoclonal antibodies (FB1 and FB21) useful for the identification of human B lymphocytes in formalin-fixed, paraffin-embedded tissues. J Pathol. 1994; 173:347-354. (Immunogen). View Reference
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Nozawa Y, Abe M, Wakasa H. Three mAb, FUN-1, FB1, and FB21, that recognize B-cell antigens in frozen or paraffin-embedded tissue sections. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:705-706.
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
555677 Rev. 6
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
