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      Purified NA/LE Rat Anti-Mouse CD44
      Purified NA/LE Rat Anti-Mouse CD44
      Blocking of hyaluronate binding by KM114 antibody. The BALB/c B-cell hybridoma BM-2 was incubated with fluorescein-conjugated hyaluronic acid (FHA) in the absence (filled histogram) or presence (open histogram) of purified anti-mouse CD44 antibody KM114 (Cat. no. 558739, at 6 µg/ml). The grey line is the profile of cells without F-HA.  Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
      Purified NA/LE Rat Anti-Mouse CD44
      Blocking of hyaluronate binding by KM114 antibody. The BALB/c B-cell hybridoma BM-2 was incubated with fluorescein-conjugated hyaluronic acid (FHA) in the absence (filled histogram) or presence (open histogram) of purified anti-mouse CD44 antibody KM114 (Cat. no. 558739, at 6 µg/ml). The grey line is the profile of cells without F-HA.  Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
      Product Details
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      BD Pharmingen™
      Pgp-1, H-CAM, Ly-24
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
      (C57BL/6 x DBA/2)F1 mouse bone marrow-derived stromal cell clone BMS2
      Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Western blot (Tested During Development), Blocking, ELISA (Reported)
      1.0 mg/ml
      AB_395009
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      553724 Rev. 8
      Antibody Details
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      KM114

      The KM114 antibody reacts with an epitope on both alloantigens and all isoforms of the CD44 glycoprotein (Pgp-1, Ly-24). The KM114 hybridoma was produced at the same time as the published clone KM201, and the mAb recognizes a different epitope from that recognized by mAb IM7. The standard form of CD44, lacking variable exons and referred to as CD44H or CD44S, is widely expressed on hematopoietic and non-hematopoietic cells. CD44 isoforms encoded by variable exons are expressed on epithelial cells, but only at low levels on most leukocytes. Mice with the Ly-24.1 alloantigen (e.g., BALB/c, CBA/J, DBA/1, DBA/2) have relatively large subsets of CD44H+ T lymphocytes, while Ly-24.2 strains (e.g., A, AKR, CBA/N, C3H/He, C57BL, C57BR, C57L, C58, NZB, SJL, SWR, 129) have lower expression of CD44H on T cells. CD44 is a cell adhesion receptor, and its ligand, hyaluronate, is a common component of extracellular matrices. Differential glycosylation of CD44 influences its binding to hyaluronate. Additional ligands include the cell-surface form of CD74 and the cytokine osteopontin (Eta-1).10 Bone marrow- and thymus-derived progenitor cells capable of repopulating the thymus express CD44.  In the periphery, the level of CD44 expression increases upon activation of B lymphocytes, CD4+ T cells, and CD8+ T cells; and memory cells can be recognized by their CD44[hi] phenotype. KM114 antibody can be used in ELISA to detect soluble CD44, and it is effective for in vitro blocking of hyaluronate recognition by CD44. It has been reported that KM114 mAb cross-reacts with CD44 of the Chinese hamster, Cricetulus griseus, but not human CD44.

      553724 Rev. 8
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      553724 Rev.8
      Citations & References
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      Development References (16)

      1. Bendelac A. Mouse NK1+ T cells. Curr Opin Immunol. 1995; 7(3):367-374. (Biology). View Reference
      2. Castro A, Bono MR, Simon V, Vargas L, Rosemblatt M. Spleen-derived stromal cells. Adhesion molecules expression and lymphocyte adhesion to reticular cells. Eur J Cell Biol. 1997; 74(4):321-328. (Biology). View Reference
      3. Godfrey DI, Kennedy J, Suda T, Zlotnik A. A developmental pathway involving four phenotypically and functionally distinct subsets of CD3-CD4-CD8- triple-negative adult mouse thymocytes defined by CD44 and CD25 expression. J Immunol. 1993; 150(10):4244-4252. (Biology). View Reference
      4. Kanamori Y, Ishimaru K, Nanno M, . Identification of novel lymphoid tissues in murine intestinal mucosa where clusters of c-kit+ IL-7R+ Thy1+ lympho-hemopoietic progenitors develop. J Exp Med. 1996; 184(4):1449-1459. (Biology). View Reference
      5. Katoh S, McCarthy JB, Kincade PW. Characterization of soluble CD44 in the circulation of mice. Levels are affected by immune activity and tumor growth. J Immunol. 1994; 153(8):3440-3449. (Clone-specific: ELISA). View Reference
      6. Katoh S, Zheng Z, Oritani K, Shimozato T, Kincade PW. Glycosylation of CD44 negatively regulates its recognition of hyaluronan. J Exp Med. 1995; 182(2):419-429. (Clone-specific: Blocking). View Reference
      7. Lesley J, Hyman R, Kincade PW. CD44 and its interaction with extracellular matrix. Adv Immunol. 1993; 54:271-335. (Biology). View Reference
      8. Lynch F, Ceredig R. Mouse strain variation in Ly-24 (Pgp-1) expression by peripheral T cells and thymocytes: implications for T cell differentiation. Eur J Immunol. 1989; 19(2):223-229. (Biology). View Reference
      9. MacDonald HR, Budd RC, Cerottini JC. Pgp-1 (Ly 24) as a marker of murine memory T lymphocytes. Curr Top Microbiol Immunol. 1990; 159:97-109. (Biology). View Reference
      10. Miyake K, Medina KL, Hayashi S, Ono S, Hamaoka T, Kincade PW. Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures. J Exp Med. 1990; 171(2):477-488. (Immunogen). View Reference
      11. Murakami S, Miyake K, June CH, Kincade PW, Hodes RJ. IL-5 induces a Pgp-1 (CD44) bright B cell subpopulation that is highly enriched in proliferative and Ig secretory activity and binds to hyaluronate. J Immunol. 1990; 145(11):3618-3627. (Biology). View Reference
      12. Naor D, Sionov RV, Ish-Shalom D. CD44: structure, function, and association with the malignant process. Adv Cancer Res. 1997; 71:241-319. (Biology). View Reference
      13. Naujokas MF, Morin M, Anderson MS, Peterson M, Miller J. The chondroitin sulfate form of invariant chain can enhance stimulation of T cell responses through interaction with CD44. Cell. 1993; 74(2):257-268. (Biology). View Reference
      14. Sprent J, Tough DF. Lymphocyte life-span and memory. Science. 1994; 265(5177):1395-1400. (Biology). View Reference
      15. Weber GF, Ashkar S, Glimcher MJ, Cantor H. Receptor-ligand interaction between CD44 and osteopontin (Eta-1). Science. 1996; 271(5248):509-512. (Biology). View Reference
      16. Zheng Z, Katoh S, He Q, et al. Monoclonal antibodies to CD44 and their influence on hyaluronan recognition. J Cell Biol. 1995; 130(2):485-495. (Biology). View Reference
      View All (16) View Less
      553724 Rev. 8

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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