Purified Hamster Anti-Mouse H2-M3

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BD Pharmingen™ Purified Hamster Anti-Mouse H2-M3

Name: Purified Hamster Anti-Mouse H2-M3
Brand: BD Pharmingen™
SKU: 551769

Clone 130

(RUO)

Upregulation of H2-M3 expression by exogenous N-formylated peptide. C57BL/6 splenocytes were cultured overnight in the absence (left panel) or presence (right panel) of N-Formyl-Met-Leu-Phe-Phe peptide (Sigma-Aldrich). The cells were then stained with either purified hamster anti- mouse H2-M3 mAb (clone 130) (open histograms) or purified hamster IgG1, κ isotype control mAb (clone A19-3) (Cat. No. 553969, filled histograms), in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32, mAb (clone 2.4G2) (Cat. No. 553141, open and filled histograms), followed by PE mouse anti-hamster IgG cocktail (Cat. No. 554056, open and filled histograms). Flow cytometry was performed on a BD FACSCalibur™ instrument.

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Product Details

Brand: BD Pharmingen™

Reactivity: Mouse (QC Testing)

Isotype: Armenian Hamster IgG

Immunogen: Purified soluble complex of recombinant truncated M3 and β2-microglobulin

Application: Flow cytometry (Routinely Tested), Immunoprecipitation (Reported)

Concentration: 0.5 mg/ml

RRID: AB_394243

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

Size 0.1 mg Cat No. 553141

Purified Hamster IgG1, κ Isotype Control RUO

Size 0.5 mg Cat No. 553969

PE Mouse Anti-Armenian and Syrian Hamster IgG Cocktail RUO

Size 0.2 mg Cat No. 554056

551769 Rev. 2

Antibody Details

130

The hamster anti-mouse H2-M3 antibody (clone 130) reacts with the H2-M3 major histocompatibility complex (MHC) non-classical class Ib antigen. H2-M3 (M3) associates with β2-microglobulin and is capable of being expressed by most leukocytes. However, due to a lack of endogenous antigens, M3 has been reported to be undetectable on most cells. Its expression is induced by high-affinity N-formylated peptides from mitochondria, Listeria monocytogenes, and Mycobacterium tuberculosis. The induced expression of M3 is most efficient on antigen presenting cells. M3 presents antigen to cytotoxic T lymphocytes and may play a key role in protective immunity against the intracellular bacteria L. monocytogenes and M. tuberculosis. Furthermore, M3 is capable of presenting mitochondrial antigens for intrathymic positive selection of T-cell receptors which recognize those peptides.

551769 Rev. 2

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

551769 Rev.2

Citations & References

Development References (6)

Berg RE, Princiotta MF, Irion S, Moticka JA, Dahl KR, Staerz UD. Positive selection of an H2-M3 restricted T cell receptor. Immunity. 1999; 11(1):33-43. (Biology). View Reference
Chiu NM, Chun T, Fay M, Mandal M, Wang CR. The majority of H2-M3 is retained intracellularly in a peptide-receptive state and traffics to the cell surface in the presence of N-formylated peptides. J Exp Med. 1999; 190(3):423-434. (Immunogen: Immunoprecipitation). View Reference
Chun T, Serbina NV, Nolt D, et al. Induction of M3-restricted cytotoxic T lymphocyte responses by N-formylated peptides derived from Mycobacterium tuberculosis. J Exp Med. 2001; 193(10):1213-1220. (Biology). View Reference
Lindahl KF, Byers DE, Dabhi VM, et al. H2-M3, a full-service class Ib histocompatibility antigen. Annu Rev Immunol. 1997; 15:851-879. (Biology). View Reference
Seaman MS, Perarnau B, Lindahl KF, Lemonnier FA, Forman J. Response to Listeria monocytogenes in mice lacking MHC class Ia molecules. J Immunol. 1999; 162(9):5429-5436. (Biology). View Reference
Shawar SM, Vyas JM, Rodgers JR, Rich RR. Antigen presentation by major histocompatibility complex class I-B molecules. Annu Rev Immunol. 1994; 12:839-880. (Biology). View Reference

View All (6)

551769 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.