Skip to main content Skip to navigation
Purified Rat Anti-Mouse CD105
Purified Rat Anti-Mouse CD105
Immunohistochemical staining of CD105 on mouse splenocytes. Frozen sections of normal mouse spleen were stained with Purified Rat Anti-Mouse CD105 (Cat. No. 550546). Visualization of CD105 was carried out with Biotin Goat Anti-Rat Ig (Cat. No. 559286) and Streptavidin HRP (Cat. No. 550946). (Magnification 20x)
Immunohistochemical staining of CD105 on mouse splenocytes. Frozen sections of normal mouse spleen were stained with Purified Rat Anti-Mouse CD105 (Cat. No. 550546). Visualization of CD105 was carried out with Biotin Goat Anti-Rat Ig (Cat. No. 559286) and Streptavidin HRP (Cat. No. 550946). (Magnification 20x)
Product Details
Down Arrow Up Arrow


BD Pharmingen™
Endoglin; Edg; EGLN; Eng; MJ7/18 antigen; S-endoglin
Mouse (QC Testing)
Rat IgG2a, κ
Mouse skin (inflamed)
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
AB_2277916
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Immunohistochemistry:  Clone MJ7/18 was tested in development for immunohistochemical staining of acetone-fixed frozen sections.  IHC of formalin-fixed paraffin embedded sections is not recommended.  This antibody has been reported to predominantly stain vascular endothelial cells. The isotype control recommended for use with this antibody is Purified Rat IgG2a κ Isotype Control (Cat. No. 559073).  For optimal indirect immunohistochemical staining, the MJ7/18 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with Biotin Goat Anti-Rat Ig (Cat. No. 559286) as the secondary antibody and streptravidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550546 Rev. 2
Antibody Details
Down Arrow Up Arrow
MJ7/18

The MJ7/18 monoclonal antibody specifically binds to mouse CD105 (also known as endoglin) which is a homodimer of 90-kDa subunits and is predominantly expressed on vascular endothelial cells.  High levels of mouse endoglin mRNA have been reported to be detectable in the ovary, uterus, NCTC-2071 fibroblasts, and to a lesser extent, in heart, muscle and stromal cells in connective tissue of various organs.  Endoglin has been reported to play an essential role in embryonic angiogenesis.  Both mouse and human endoglin display strong amino-acid sequence homology to the transmembrane and cytoplasmic regions of the type III TGF-ß receptor.

550546 Rev. 2
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550546 Rev.2
Citations & References
Down Arrow Up Arrow

Development References (3)

  1. Ge AZ, Butcher EC. Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand. Gene. 1994 January; 138(1-2):201-206. (Immunogen). View Reference
  2. Li DY, Sorensen LK, Brooke BS. Defective angiogenesis in mice lacking endoglin. Science. 1999; 284(5419):1534-1537. (Biology). View Reference
  3. St-Jacques S, Cymerman U, Pece N, Letarte M. Molecular characterization and in situ localization of murine endoglin reveal that it is a transforming growth factor-beta binding protein of endothelial and stromal cells. Endocrinology. 1994 June; 134(6):2645-2657. (Biology). View Reference
550546 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.