BD ACCURI C6 PLUS
Many modern industries employ biological processes that require precise monitoring and control. Bacterial bioprocessing produces ingredients for food, drugs, cosmetics, and more; algae can be used to manufacture biofuels; yeast fermentation produces wine and beer. By offering rapid analysis of heterogeneous samples, flow cytometry is an ideal method for monitoring these processes.
BD Accuri™ flow cytometers offer particular advantages for industrial and agricultural applications. Compact and rugged, they can be transported into labs and processing facilities, and even into the field. The open fluidics system allows it to connect easily to bioreactors and fermentation units for automatic sampling and continuous culture monitoring.
The following sections and resources illustrate the rich data you can generate by using BD Accuri systems for industrial applications in bioprocessing, biofuels, agriculture, fermentation, and other enterprises.
Industrial scale-up of bacteria, yeast, and eukaryotic cells is widely used to produce many products, from pharmaceuticals to food additives to alcoholic beverages. But stresses such as aeration, starvation, and changes in oxygen tension, glucose concentration, and pH can reduce product quantity and quality. Precise control and optimization of cell growth are crucial.
Flow cytometry offers a powerful and effective methodology for monitoring cellular status and growth in bioreactors by rapidly characterizing complex cultures at the single-cell level. Flow cytometry applications common in bioprocessing include cell counting, viability, cell cycle, apoptosis, transfection efficiency, cell line development, and media optimization.
Offering both performance and simplicity, BD Accuri systems allow bioprocess engineers to use light scatter signals to discriminate cells and fluorescence signals to measure cell viability, vitality, and other important cell characteristics. BD Accuri systems also interface smoothly with automated bioreactor sampling systems so that samples can be taken continuously and analyzed automatically. Bioprocess engineers can use the resulting real-time, online bioreactor data for pinpoint process control.
- White Paper: Bioprocess Monitoring with the BD Accuri™ Flow Cytometer
- White Paper: Automating the Personal Flow Cytometer
- Technical Bulletin: Baculovirus Titration with the BD Accuri™ Flow Cytometer
- BioTechniques Webinar Replay: Obtaining Multi-Parametric, Real-Time Measurements in Industrial Bioprocessing Using Automated Flow Cytometry
- Customer Interview: Frank Delvigne on Online Bioprocess Monitoring
- Customer Interview: Tim Overton on Recombinant Protein Production and Fermentation Analysis
Some authorities argue that algal biofuel is the best renewable candidate to fully replace fossil petroleum. Research laboratories racing to find or develop strains of microalgae suitable for biofuel development need efficient analytical tools to monitor algal cultures—rapidly and in real time—for size, growth, viability, chlorophyll autofluorescence, and lipid content.
Flow cytometry offers a powerful and effective method for screening microalgal cultures, and BD Accuri systems are especially well suited to the task. Microalgae can vary greatly in size, can live as single cells or in colonies, and cultures grown in open ponds often contain debris and other contaminating organisms. These characteristics can make them difficult to process through a flow cytometer’s fluidics system, even when pond water is filtered before acquisition. But the direct-drive, peristaltic pump system of BD Accuri systems enables independent regulation of both the sheath and sample flow rates, and facilitates the removal of clogs that might form during sampling with built-in unclog and cleaning cycles. Using lipophilic dyes, BD Accuri systems can rapidly estimate neutral lipid storage in small samples of live algal cells, usually without needing to remove the alga from its growth medium.
- White Paper: Multiparametric Analysis of Microalgae for Biofuels Using Flow Cytometry
- Customer Interview: Rose Ann Cattolico and James Barker on Algal Biofuel Research
Of the approximately 500,000 species of angiosperms (flowering plants) in the world, only about 2% have been described even minimally at the molecular level. Because anthropogenic change is driving many species extinct each year, a global molecular and genomic census of angiosperms is needed.
Flow cytometry can provide a rapid and efficient way of quantifying DNA content using dyes such as propidium iodide (PI). However, plant nuclear DNA content (ploidy) varies over extreme ranges, and nuclear DNA measurements of plants are often hampered by excessive cellular and subcellular debris and autofluorescence from other prevalent cellular components, such as chloroplasts.
Several factors make BD Accuri systems ideal for determining the nuclear DNA contents of plants. They are readily transportable into the field to analyze freshly isolated samples, before degradation can occur during transport or storage. The sophisticated digital signal processing system has a seven-decade dynamic range that encompasses almost the entire known span of plant C-values (haploid nuclear DNA content). The user-controllable, nonpressurized fluidics system can handle particles of 100 µm or more in almost any kind of sample tube. Finally, a blue laser optimally excites PI. A high correlation between PI-DNA emission in the FL2 and FL3 channels places the nuclear events in a narrow diagonal region from which it is easy to exclude fragments and debris.
- White Paper: Using the BD Accuri™ Flow Cytometer for Rapid and Accurate Analysis of the Nuclear DNA Contents of Flowering Plants
- Article: Determining DNA Content of Plant (and Other) Tissues
- Genetic Engineering & Biotechnology News Webinar Replay: Using Flow Cytometry to Speed Determination of Eukaryotic Genome Sizes and Gene Expression
- Customer Interview: David Galbraith on Plant Genome Analysis
Food, Beverage, and Veterinary
In industrial processes, microbes can represent contaminants to be controlled or beneficial agents to be encouraged. In the first two interviews below, researchers describe how they use BD Accuri systems to detect foodborne pathogens and monitor fermentations. In the third, a team describes how they use flow cytometry to optimize cryopreservation of semen in their efforts to preserve the endangered Asian elephant.
All reagents and kits are compatible with both the BD Accuri C6 Plus and BD Accuri C6 flow cytometer systems. Platforms referred to as "BD Accuri" represent both the BD Accuri C6 Plus and BD Accuri C6. Data was generated on either the BD Accuri C6 Plus or the BD Accuri C6 as indicated in figure legends.