BD ACCURI C6 PLUS

Education

Education - Student Photo

Many research and teaching institutions introduce graduate students to flow cytometry as part of their training and research. Increasingly, however, research professors and other academic instructors report that they are including active flow cytometry usage in their undergraduate curricula. Direct observation and experimentation are widely becoming central to a strong undergraduate education in biology as well as other disciplines.

Personal, approachable, and unintimidating, BD Accuri™ flow cytometers are ideal for both graduate and undergraduate usage. Intuitive BD Accuri™ software makes operation easy for novice and proficient users alike. The compact size and rugged design make it easy to transport into classrooms and teaching labs without optical realignment. And the low cost makes it an attractive option for individual research labs and smaller educational institutions.

Beyond coursework, undergraduates at these institutions also participate in a wide variety of research projects involving flow cytometry. Topics include lymphocyte biology, cell differentiation and development, cell culture, oxidative stress, calcium flux, metabolism, cell cycle analysis, and apoptosis and cell death. The ability to perform hands-on research using BD Accuri systems helps these young scientists understand what it's like to work in a modern research lab. And if they decide to pursue a research career, their flow cytometry experience makes them more competitive for acceptance into top-tier graduate and professional schools.

Resources

Sample Data

Flow cytometry immunophenotyping lab in an undergraduate immunology course
Flow cytometry immunophenotyping lab in an undergraduate immunology course

In Melanie Gubbels Bupp's undergraduate immunology lab at Randolph-Macon College, students perform a three-lab experiment on leucocyte subset infiltration during thioglycollate-induced peritonitis.

Lab Session I:

  • Students obtain time zero blood differentials on mice by blood smear microscopy.
  • The instructor injects mice with thioglycollate to induce peritonitis.

Lab Session II:

  • Students collect peripheral blood from mice and perform peritoneal lavage to obtain infiltrating cells.
  • Students obtain blood differentials on peripheral blood smears.
  • Students perform cell staining with an antibody panel designed to differentiate various white blood cell (WBC) populations (see figure).
  • Cells are fixed and held for up to 1 week, covered and refrigerated.

Lab Session III:

  • Students collect data on the BD Accuri C6 flow cytometer using an instructor-created template.
  • The instructor uploads FC plots and population percentage information to the course website.
  • Students calculate and graph the WBC population numbers with time for both the peripheral blood and peritoneal lavage data.
An undergraduate experiment on compound research effects at CSU Northridge
An undergraduate experiment on compound research effects at CSU Northridge
Student groups at California State University, Northridge, cultured CEM-C7-14 leukemia cells at a designated cell density. Each group received a different compound or compound concentration and added it to the culture. Later, the students stained cells with propidium iodide, which stoichiometrically binds DNA, and collected and analyzed data on a BD Accuri C6. Results: Top row: CEM-C7-14 cells cultured with a compound for various durations. Bottom row: Control cells cultured with EtOH. Compound exposure induces cell death as indicated by the rise in sub-G0/G1 events over time compared to controls. Data courtesy of Dr. Rheem D. Medh, California State University, Northridge, CA.



All reagents and kits are compatible with both the BD Accuri C6 Plus and BD Accuri C6 flow cytometer systems. Platforms referred to as "BD Accuri" represent both the BD Accuri C6 Plus and BD Accuri C6. Data was generated on either the BD Accuri C6 Plus or the BD Accuri C6 as indicated in figure legends.