Multicolor Flow Cytometry



Ultraviolet Laser Overview

Fluorochromes

 

BD Horizon™ BUV395

BD Horizon Brilliant™ Ultraviolet 395 (BUV395) (Ex Max 348 nm/Em Max 395 nm) is a UV-excitable dye that has been developed exclusively by BD Biosciences for instruments equipped with a 355-nm UV laser. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.

Filter: 379/28
Relative brightness: Moderate (on a scale of Dim, Moderate, Bright, and Brightest)
Sample data:

Lysed whole blood stained with CD4 or CD56 in BUV395 or FITC formats
Lysed whole blood stained with CD4 or CD56 in BUV395 or FITC formats
Data shown was gated on lymphocytes. BUV395 was excited by a 355-nm laser and FITC was excited by a 488-nm laser.

 
 

BD Horizon™ BUV496

BD Horizon Brilliant™ Ultraviolet 496 (BUV496) (Ex Max 348 nm/Em Max 496 nm) is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission maximum at 496 nm. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon™ V500 or BV510 (for example, 525/40-nm filter). BUV496 has been exclusively developed by BD Biosciences for instruments equipped with a 355-nm UV laser.

Filter: 515/30
Relative brightness: Moderate (on a scale of Dim, Moderate, Bright, and Brightest)
Sample data:

Lysed whole blood stained with CD4 or CD19 in BUV496 or FITC formats.
Lysed whole blood stained with CD4 or CD19 in BUV496 or FITC formats.
Data shown was gated on lymphocytes. BUV496 was excited by a 355-nm laser and FITC was excited by a 488-nm laser.

 
 

BD Horizon™ BUV661

BD Horizon Brilliant™ Ultraviolet 661 (BUV661) (Ex Max 348 nm/Em Max 661 nm) is a tandem dye that combines BUV395 and an acceptor dye with an emission maximum at 661 nm. As part of the BD Horizon Brilliant Ultraviolet family, BUV661 provides an additional option for multicolor panels utilizing UV excitable dyes.

Filter: 670/25, 630nm
Relative brightness: Bright (on a scale of Dim, Moderate, Bright, and Brightest)
Sample data:

Lysed whole blood stained with Hu CD3 or HU CD11c BUV661 and appropriate isotype control.
Lysed whole blood stained with Hu CD3 or HU CD11c BUV661 and appropriate isotype control.
Data shown on lymphocytes.
Mouse splenocytes stained with Ms CD3e Alexa Fluor® 488 and Ms CD19 BUV661.
Mouse splenocytes stained with Ms CD3e Alexa Fluor® 488 and Ms CD19 BUV661.
Mouse bone-marrow cells stained with Ms CD11b BUV661.
Mouse bone-marrow cells stained with Ms CD11b BUV661.

 
 

BD Horizon™ BUV737

BD Horizon Brilliant™ Ultraviolet 737 (BUV737) (Ex Max 348 nm/Em Max 737 nm) is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission maximum at 737 nm. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting dyes with properties like Alexa Fluor® 700 (for example, 712/20-nm filter). BUV737 has been exclusively developed by BD Biosciences for instruments equipped with a 355-nm UV laser.

Filter: 740/35
Relative brightness: Bright (on a scale of Dim, Moderate, Bright, and Brightest )
Sample data:

Lysed whole blood stained with CD19 or CD127 in BUV737 or FITC formats
Lysed whole blood stained with CD19 or CD127 in BUV737 or FITC formats
Data shown was gated on lymphocytes. BUV737 was excited by a 355-nm laser and FITC was excited by a 488-nm laser.

 
 

BD Horizon™ BUV805

BD Horizon Brilliant™ Ultraviolet 805 (BUV805) (Ex Max 348 nm/Em Max 805 nm) is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission maximum at 805 nm. BUV805 has been exclusively developed by BD Biosciences for instruments equipped with a 355-nm UV laser.

Filter: 820/60
Relative brightness: Dim (on a scale of Dim, Moderate, Bright, and Brightest)
Sample data:

Lysed whole blood stained with CD4 or CD20 BUV805 and appropriate isotype control.
Lysed whole blood stained with CD4 or CD20 BUV805 and appropriate isotype control.
Data shown on lymphocytes. b. Mouse splenocytes stained with CD3e APC and CD4 or CD8a BUV805.

 



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