BD Pharmingen™ FITC Mouse Anti-Human NPM-ALK/ALK Set
(RUO)
Description
Reacts with NPM-ALK, a chimeric gene product of approximately 80 kDa, which is the result of t(2;5)(p23;q35) chromosomal translocation. The gene at 5q35 encodes for the cell cycle-regulated nucleolar protein, nucleophosmin (NPM), while the expression of NPM-ALK protein, or p80, comprised of the N-terminal portion of NPM (about 40% of the molecule) linked to the entire intracellular portion of ALK. ALK1 antibody recognizes a formalin resistant epitope in both the NPM-ALK chimeric and the 200 kDa normal human ALK proteins. Cytoplasmic and nuclear expression of NPM-ALK is seen in t(2;5)+ cell lines Su-DHL-1 and Karpas 299.
Preparation And Storage
Recommended Assay Procedures
We recommend to use the Cytofix/cytoperm solution (Cat. No. 554714) for the cell fixation and permeabilization. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| FITC-Mouse Anti-Human NPM-ALK | 100 Tests (1 ea) | 51-37594X | N/A |
| FITC Mouse IgG3 Kappa Isotype Control | 100 Tests (1 ea) | 51-35434X | N/A |
Development References (3)
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Morris SW, Kirstein MN, Valentine MB, et al. Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin's lymphoma. Science. 1994; 263(5151):1281-1284. (Biology). View Reference
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Pittaluga S, Wlodarska I, Pulford K, et al. The monoclonal antibody ALK1 identifies a distinct morphological subtype of anaplastic large cell lymphoma associated with 2p23/ALK rearrangements. Am J Pathol. 1997; 151(2):343-351. (Biology). View Reference
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Pulford K, Lamant L, Morris SW, et al. Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1. Blood. 1997; 89(4):1394-1404. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
