Analysis of MSI1 staining in H9 human embryonic stem (ES) cells and H9-derived Neural Stem Cells (NSC). H9 human ES cells (left panel, WiCell, Madison, WI) and H9-derived NSC (right panel) were harvested, fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD™ Phosflow Perm buffer III (Cat. No. 558050) and stained with PE Mouse Anti-Human MSI1 antibody (open histograms) or a matching concentration of a PE Mouse IgG1, κ isotype control (shaded histograms, Cat. No. 554680). Use of the BD™ Phosflow Perm/Wash buffer I (Cat. No. 557885) is not recommended. The histograms were derived from gated events based on light scattering characteristics for the H9 cell line (left panel) or NSC (right panel). Flow cytometry was performed on a BD LSR™ II flow cytometry system.
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Analysis of MSI1 staining in H9 human embryonic stem (ES) cells and H9-derived Neural Stem Cells (NSC). H9 human ES cells (left panel, WiCell, Madison, WI) and H9-derived NSC (right panel) were harvested, fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD™ Phosflow Perm buffer III (Cat. No. 558050) and stained with PE Mouse Anti-Human MSI1 antibody (open histograms) or a matching concentration of a PE Mouse IgG1, κ isotype control (shaded histograms, Cat. No. 554680). Use of the BD™ Phosflow Perm/Wash buffer I (Cat. No. 557885) is not recommended. The histograms were derived from gated events based on light scattering characteristics for the H9 cell line (left panel) or NSC (right panel). Flow cytometry was performed on a BD LSR™ II flow cytometry system.
Product Details
BD Pharmingen™
Musashi-1, RNA-binding protein Musashi homolog 1, MSI1H
Human (QC Testing), Rat (Predicted)
Mouse IgG1, κ
Human MSI1 a.a. 221-311 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
AB_10689629
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
Perm Buffer III RUO
Size
125 mL
Cat No.
558050
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
561468 Rev. 1
Antibody Details
N14-47
The N14-47 monoclonal antibody recognizes RNA-binding protein Musashi homolog 1 (Musashi-1 or MSI1) that represses the translation of particular mRNAs by binding specific RNA sequence motifs. MSI1 contains two RNA-recognition domains at amino acids 20-110 and 109-186. It is expressed in early lineage cells, such as neural stem/progenitor cells, small intestinal stem cells, and mammary stem cells. MSI1 has also been detected in many human cancers, including gliomas, melanomas, colorectal adenomas and adenocarcinomas, suggesting its involvement in cancer development.
561468 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
561468 Rev.1
Citations & References
Development References (3)
-
Glazer RI, Wang XY, Yuan H, Yin Y. Musashi1: a stem cell marker no longer in search of a function. Cell Cycle. 2008; 7(17):2635-2639. (Biology). View Reference
-
Montgomery RK, Breault DT. Small intestinal stem cell markers. J Anat. 2008; 213(1):52-58. (Biology). View Reference
-
Okano H, Imai T, Okabe M. Musashi: a translational regulator of cell fate. J Cell Sci. 2002; 115:1355-1359. (Biology). View Reference
561468 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
