Skip to main content Skip to navigation
Hamburger Menu
Search icon
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
United States (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified Mouse Anti-Human CD19
      Product Details
      Down Arrow Up Arrow


      BD™
      B4; B-lymphocyte antigen CD19; Leu-12; Leu12
      Human
      Mouse BALB/c IgG1, κ
      Human Chronic Lymphocytic Leukemia (CLL) Cells
      Flow cytometry
      25 μg/mL
      20 μL
      II B43
      930
      Phosphate buffered saline with gelatin and 0.1% sodium azide.
      RUO (GMP)


      Preparation And Storage

      The monoclonal antibody is supplied as 50 μg purified immunoglobulin in 2.0 mL (25 μg/mL) of phosphate-buffered saline (PBS). The FITC conjugate is supplied as 50 μg in 2.0 mL (25 μg/mL). The PE conjugate is supplied as 25 μg/mL in 1.0 mL. The PerCP conjugate is supplied as 12.5 μg/mL in 2.0 mL (6.25 μg/mL). PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

      Show More blue down arrow Show Less blue up arrow
      347540 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      4G7

      The CD19 antibody, clone 4G7, is derived from hybridization of P3-X63-Ag8.653 mouse cells with spleen cells from BALB/c mice immunized with human chronic lymphocytic leukemia (CLL) cells.

      The CD19 (Leu-12) antibody recognizes a human B-lymphocyte antigen, with a molecular weight of 90 kilodaltons (kDa).

      347540 Rev. 1
      Format Details
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      347540 Rev.1
      Citations & References
      Down Arrow Up Arrow

      Development References (8)

      1. Bloomfield C, Gajl-Peczalska K, Frizzera G, Kersey J, Goldman A. Clinical utility of surface markers combined with Lukes-Collins histologic classification in adult lymphoma. N Eng J Med. 1979; 301:512. (Biology).
      2. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:34-36.
      3. Foucar K, Goeken JA. Clinical application of immunologic techniques to the diagnosis of lymphoproliferative and immunodeficiency disorders. Lab Med. 1982; 13:403-413. (Biology).
      4. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. Blood. 1987; 70(5):1316-1324. (Biology). View Reference
      5. Meeker TC, Miller RA, Link MP, Bindl J, Warnke R, Levy R. A unique human B lymphocyte antigen defined by a monoclonal antibody.. Hybridoma. 1984; 3(4):305-20. (Biology). View Reference
      6. Moldenhauer G, Dörken B, Schwartz R, Pezzutto A, Knops J, Hammerling GJ. Analysis of ten B lymphocyte-specific workshop monoclonal antibodies. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:61-67.
      7. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
      8. Warnke RA, Link MD. Identification and significance of cell markers in leukemia and lymphoma. Ann Rev Med. 1983; 34:117. (Biology).
      View All (8) View Less
      347540 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

      Website Feedback