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Mouse IL-5 Enhanced Sensitivity Flex Set

BD™ Cytometric Bead Array (CBA) Mouse IL-5 Enhanced Sensitivity Flex Set

(RUO)
Mouse IL-5 Enhanced Sensitivity Flex Set
Figure 1. Example of BD CBA Mouse IL-5 Enhanced Sensitivity Flex Set standard curve. Data was acquired using a BD FACSArray™ Bioanalyzer and analyzed using FCAP Array Software (Cat. No. 641488).
Mouse IL-5 Enhanced Sensitivity Flex Set
Linearity: Cell culture supernatant and 1:3 diluted pooled mouse serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.
Mouse IL-5 Enhanced Sensitivity Flex Set
Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Mouse IL-5 Enhanced Sensitivity Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay) respectively.
Mouse IL-5 Enhanced Sensitivity Flex Set
Recovery: Cell culture supernatant and pooled mouse serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:3 before the protein was spiked into each. Serum used was commercially available pooled mouse serum.
Figure 1. Example of BD CBA Mouse IL-5 Enhanced Sensitivity Flex Set standard curve. Data was acquired using a BD FACSArray™ Bioanalyzer and analyzed using FCAP Array Software (Cat. No. 641488).
Linearity: Cell culture supernatant and 1:3 diluted pooled mouse serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.
Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Mouse IL-5 Enhanced Sensitivity Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay) respectively.
Recovery: Cell culture supernatant and pooled mouse serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:3 before the protein was spiked into each. Serum used was commercially available pooled mouse serum.
Product Details
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BD™ Cytometric Bead Array (CBA)
A6
274-200,000 fg/mL
Mouse (QC Testing)
RUO
AB_2869402


Description

The BD™ CBA Mouse IL-5 Enhanced Sensitivity Flex Set is a bead-based immunoassay capable of measuring mouse Interleukin-5 (IL-5) in serum and cell culture supernatant samples. The BD CBA Enhanced Sensitivity Flex Sets are capable of measuring soluble analytes present in very low concentrations. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Mouse Enhanced Sensitivity Master Buffer Kit (Cat. No. 562246 or 562248).

Preparation And Storage

This BD CBA Enhanced Sensitivity Flex Set contains one vial each of Enhanced Sensitivity Capture Bead and Detection Reagent (Part A) and two vials of Standard. The Enhanced Sensitivity Detection Reagent (Part B) is provided in the BD CBA Mouse Enhanced Sensitivity Master Buffer Kit. The Enhanced Sensitivity Capture Bead and Detection Reagent (Part A) components of this flex set have been formulated to a 20x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and when reconstituted according to the instructions in the BD CBA Mouse Enhanced Sensitivity Master Buffer kit, the top standard point for this assay is 200,000 fg/ml. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C.

Recommended Assay Procedures

The BD CBA Mouse IL-5 Enhanced Sensitivity Flex Set must be used in conjunction with a BD CBA Mouse Enhanced Sensitivity Master Buffer Kit (Cat. No. 562246, 100 tests, or 562248, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD CBA Mouse Enhanced Sensitivity Master Buffer Kit. When following the directions in the Master Buffer Kit, the standard range for the BD CBA Mouse IL-5 Enhanced Sensitivity Flex Set will be 274 to 200,000 fg/ml. An example standard curve is shown in Figure 1.

The BD CBA Human IL-5 Enhanced Sensitivity Flex Set should only be used in the same assay well with other BD CBA Mouse Enhanced Sensitivity Flex Set reagents. We do not recommend plexing Enhanced Sensitivity Flex Set assays along with assays from any other Flex Set system (soluble protein, cell signaling, etc.). For an updated assay compatibility chart for the BD CBA Mouse Enhanced Sensitivity Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/cbasetup.

Performance

Limit of Detection: The theoretical limit of detection is 12.5 fg/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 fg/ml, n=30) + 2 standard deviations.

Product Notices

  1. ProClin is a trademark of Rohm and Haas Company.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Warning: CBA lyophilized standard contains 0.02% (w/w) and Detection Reagent (Part A) contains 0.002% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.
562234 Rev. 2
Components
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Description Quantity/Size Part Number EntrezGene ID
Mouse IL-5 Standard 1 Vial(s) (2 ea) 51-9003524 N/A
Mouse IL-5 Enhanced Sensitivity Capture Bead A6 N/A 51-9007636 N/A
Mouse IL-5 Detection Reagent (Part A) N/A 51-9007637 N/A
562234 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.