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      IMK-Lymphocyte

      BD Simultest™ IMK-Lymphocyte

      (IVD)
      Product Details
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      BD Simultest™
      Human
      Flow cytometry
      IVD


      Description

      Becton Dickinson Simultest™ IMK-Lymphocyte is a two-color direct immunofluorescence reagent kit for enumerating percentages of the following mature human leucocyte subsets in erythrocyte-lysed whole blood (LWB): T (CD3+) lymphocytes, B (CD19+) lymphocytes, helper/inducer T (CD3+CD4+) lymphocytes, suppressor/cytotoxic T (CD3+CD8+) lymphocytes, and natural killer (NK) (CD3–CD16+and/or CD56+) lymphocytes. The helper/suppressor T-lymphocyte ratio (CD3+CD4+/CD3+CD8+) can also be determined.

      Contents: BD FACS™ Lysing Solution and 6 BD Simultest™ reagents:BD Leucogate™ (CD45 FITC / CD14 PE), Isotype Control (IgG1 FITC / IgG2a PE), CD3 FITC / CD19 PE, CD3 FITC / CD4 PE, CD3 FITC / CD8 PE, and CD3 FITC / CD16 PE + CD56 PE.

      Preparation And Storage

      1. For in vitro diagnostic use.

      2. When stored at 2° to 8°C, antibody reagents are stable until the expiration date shown on the label. Do not use after the expiration date.

      3. The antibody reagents should not be frozen or exposed to direct light during storage or during incubation with cells. Keep the reagent vial dry.

      4. Incubation or centrifugation times or temperatures other than those specified can be a source of error.

      5. For optimal results, stain blood samples within 6 hours of venipuncture.

      6. Alteration in the appearance of any of the reagents, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.

      7. The antibody reagents contain sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which can cause erroneous results.

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      340182 Rev. 1
      Components
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      Description Quantity/Size Part Number EntrezGene ID
      BD Leucogate™ (CD45 FITC / CD14 PE) 50 Tests (1 ea) 91-0168PR N/A
      Isotype Control (IgG1 FITC / IgG2a PE) 50 Tests (1 ea) 91-0169PR N/A
      BD Simultest™ CD3 FITC / CD19 PE 50 Tests (1 ea) 91-0170PR N/A
      BD Simultest™ CD3 FITC / CD4 PE 50 Tests (1 ea) 91-0171PR N/A
      BD Simultest™ CD3 FITC / CD8 PE 50 Tests (1 ea) 91-0172PR N/A
      BD Simultest™ CD3 FITC / CD16 PE + CD56 PE 50 Tests (1 ea) 91-0173PR N/A
      BD FACS™ Lysing Solution 50 Tests (1 ea) 91-0174 N/A
      340182 Rev. 1
      Citations & References
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      340182 Rev. 1

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      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For In Vitro Diagnostics Use.

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