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      CD3 FITC/CD19 PE/CD45 PerCP

      BD Tritest™ CD3 FITC/CD19 PE/CD45 PerCP

      (IVD)
      Product Details
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      BD Tritest™
      Human
      Flow cytometry
      IVD
      Buffered saline with BSA and 0.1% sodium azide.


      Description

      BD Tritest™ CD3 fluorescein isothiocyanate (FITC)/CD19 phycoerythrin (PE)/CD45 peridinin chlorophyll protein (PerCP) is a three-color direct immunofluorescence reagent for use with a suitably equipped flow cytometer to identify and determine the percentages and absolute counts of mature human T (CD3+) and B (CD19+) lymphocytes in erythrocytelysed whole blood. When used with Trucount™ Tubes, absolute counts of these populations can be enumerated from a single tube. BD Tritest reagent and Trucount Tubes can be used with the FACS Loader. The reagent can be used with or without an isotype control.

      Preparation And Storage

      1. Store the reagent at 2–8°C. Do not use after the expiration date shown on the label.

      2. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the reagent vial dry.

      3. Store TruCOUNT Tubes in their original foil pouch at 2–25°C. To avoid potential condensation, open the pouch only after it has reached room temperature and carefully reseal the pouch immediately after removing a tube. Examine the desiccant each time you open the pouch. If the desiccant has turned from blue to lavender, discard the remaining tubes. Use tubes within 1 hour after removal from the foil pouch and do not use beyond the expiration date indicated on the packaging.

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      340381 Rev. 1
      Components
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      Description Clone Isotype EntrezGene ID
      CD3 FITC SK7 IgG1, κ N/A
      CD19 PE SJ25C1 IgG1, κ N/A
      CD45 PerCP 2D1 IgG1, κ N/A
      340381 Rev. 1
      Citations & References
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      Development References (28)

      1. Berti E, Parravicini C, Cattoretti G, Delia D, de Braud F, Cusini M. Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. 1986:313-318.
      2. Brenner MB, McClean J, Dialynas DP, et al. Identification of a putative second T cell receptor. Nature. 1986; 322:145-49. (Biology).
      3. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
      4. Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988; 6:629. (Biology).
      5. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes. NCCLS document H42-T. 1992. (Biology).
      6. Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:788-803.
      7. Cohen SB, Weetman AP Activated interstitial and intraepithelial thyroid lymphocytes in autoimmune thyroid disease. Acta Endocrinol. 1988; 119:161-166. (Biology).
      8. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:34-36.
      9. Foucar K, Goeken JA. Clinical application of immunologic techniques to the diagnosis of lymphoproliferative and immunodeficiency disorders. Lab Med. 1982; 13:403-413. (Biology).
      10. Giorgi JV. Lymphocyte subset measurements: significance in clinical medicine. In: Rose NR, Friedman H, Fahey JL, ed. Manual of Clinical Laboratory Immunology. 3rd ed.. Washington, DC: American Society for Microbiology; 1986:236-246.
      11. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
      12. How to Define and Determine Reference Intervals in the Clinical Laboratory: Approved Guideline. NCCLS document C28-A. 1992. (Biology).
      13. Jackson AL, Warner NL. Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
      14. Kan EAR, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti–Leu-4 antibody. J Immunol. 1983; 131:536-539. (Biology).
      15. Knowles RW. Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II. Human T Lymphocytes. New York, NY: Springer-Verlag; 1986:259-288.
      16. Nadler LM. B Cell/Leukemia Panel Workshop: summary and comments. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:3-43.
      17. Nicholson J, Browning S, Orloff S, McDougal J. Inactivation of HIV-infected H9 cells in whole blood preparations by lysing/fixing reagents used in flow cytometry. J Immunol Methods. 1993; 160:215-218. (Biology).
      18. Nicholson J, Kidd P, Mandy F, Livnat D, Kagan J. Three-color supplement to the NIAID DAIDS guideline for flow cytometric immunophenotyping. Cytometry. 1996; 26:227-230. (Biology).
      19. Nicholson J. Use of flow cytometry in the evaluation and diagnosis of primary and secondary immunodeficiency diseases. Arch Pathol Lab Med. 1989; 113:598-605. (Biology).
      20. Nicholson JKA, Hubbard M, Jones BM. Use of CD45 fluorescence and side-scatter characteristics for gating lymphocytes when using the whole blood lysis procedure and flow cytometry. Cytometry. 1996; 26:16-21. (Biology).
      21. Nicholson JKA, Jones BM, Hubbard M. CD4 T-lymphocyte determinations on whole blood specimens using a single-tube three-color assay. Cytometry. 1993; 14:685-689. (Biology).
      22. Prince HE, Hirji K, Waldbeser LS, Plaeger-Marshall S, Kleinman S, Lanier LL. Influence of racial background on the distribution of T-cell subsets and Leu 11-positive lymphocytes in healthy blood donors. Diagn Immunol. 1985; 3(1):33-37. (Biology).
      23. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture: Approved Standard. NCCLS document H3-A3. 1991. (Biology).
      24. Protection of Laboratory Workers from Infectious Disease Transmitted by Blood, Body Fluids, and Tissue: Tentative Guideline. NCCLS document M29-T2. (Biology).
      25. Schmidt RE. Monoclonal antibodies for diagnosis of immunodeficiencies. Blut. 1989; 59:200-206. (Biology).
      26. Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
      27. Smolen J, Chused T, Leiserson W, Reeves J, Alling D, Steinberg A. Heterogeneity of immunoregulatory T-cell subsets in systemic lupus erythematosus. Am J Med. 1982; 72:783-790. (Biology).
      28. van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
      View All (28) View Less
      340381 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For In Vitro Diagnostics Use.

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