Anti-TCR-α/β FITC

BD™ Anti-TCR-α/β FITC

Name: Anti-TCR-α/β FITC
Brand: BD™
SKU: 340883

Clone WT31 (also known as WT 31; WT-31)

(ASR)

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Product Details

Brand: BD™

Alternative Name: αβ TCR; TCR alpha-beta

Reactivity: Human

Isotype: Mouse IgG1, κ

Immunogen: Human Thymocytes

Application: Flow cytometry

Concentration: 50 μg/mL

Vol. Per Test: 20 μL

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: ASR

Preparation And Storage

Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340883 Rev. 1

Antibody Details

WT31

Clone WT31 is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

WT31 (Anti–TCR-α/β-1) recognizes a conformational epitope formed by the T-cell receptor (TCR) for antigen and the CD3 epsilon chain. The α/β TCR is a disulfide-linked 80-kDalton (kDa) heterodimer consisting of a 44-kDa α chain and a 37-kDa β chain.

340883 Rev. 1

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

340883 Rev.1

Citations & References

Development References (9)

Brenner M, McClean J, Dialynas D, et al. Identification of a putative second T cell receptor. Nature. 1986; 322:145. (Biology).
Gupta S, Shimizu M, Ohira K, Vayuvegula B. T cell activation via the T cell receptor: a comparison between WT31 (defining alpha/beta TcR)-induced and anti-CD3-induced activation of human T lymphocytes.. Cell Immunol. 1991; 132(1):26-44. (Biology). View Reference
Lanier LL, Weiss A. Presence of Ti (WT31) negative T lymphocytes in peripheral blood and thymus. Nature. 1986; 324:268. (Biology).
Oettgen HC, Kappler J, Tax WJ, Terhorst C. Characterization of the two heavy chains of the T3 complex on the surface of human T lymphocytes. J Biol Chem. 1984; 259(19):12039-12048. (Biology). View Reference
Slameron A, Sanchez-Madrid F, Ursa MA, Fresno M, and Alarcon B. A conformational epitope expressed upon association of the CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies. J Immunol. 1991; 147:3047-3052. (Biology).
Spits H, Borst J, Tax W, Capel PJ, Terhorst C, de Vries JE. Characteristics of a monoclonal antibody (WT-31) that recognizes a common epitope on the human T cell receptor for antigen.. J Immunol. 1985; 135(3):1922-8. (Biology). View Reference
Tax WJ, Leeuwenberg HF, Willems HM, Capel PJ, Koene RA. Monoclonal antibodies reactive with OKT3 antigen of OKT8 antigen. In: Bernard A. A. Bernard .. et al., ed. Leucocyte typing : human leucocyte differentiation antigens detected by monoclonal antibodies : specification, classification, nomenclature = Typage leucocytaire : antigènes de différenciation leucocytaire humains révélés par les anticorps monoclonaux : "Rapports des études communes". Berlin New York: Springer-Verlag; 1984:721-722.
Tax WJ, Willems HW, Reekers PP, Capel PJ, Koene RA. Polymorphism in mitogenic effect of IgG1 monoclonal antibodies against T3 antigen on human T cells.. Nature. 304(5925):445-7. (Biology). View Reference
Weiss A, Newton M, Crommie D. Expression of T3 in association with a molecule distinct from the T-cell antigen receptor heterodimer.. Proc Natl Acad Sci USA. 1986; 83(18):6998-7002. (Biology). View Reference

View All (9)

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.