BD Accuri C6 Plus

直觀的軟體 — 幾分鐘內即可輕鬆掌握

BD CFlow® 軟體介面的設計是基於對專業流式用戶的操作要求作深入理解後而開發的,是具有簡潔扼要,直觀明瞭特徵的分析軟體。

大多數流式細胞儀使用者都會驚訝的發現CFlow是一款如此容易上手的軟體,不到一個小時的時間內,即可完成從樣本採集到分析資料的全過程。軟體的各種選項以及儀器的操控都可以從分門別類的操作介面上清晰可辨,通過這些直觀的介面結構設計,使用者可以最直接地訪問樣本採集、資料分析和統計的各個功能模組。


通過樣本採集介面獲取實驗資料。使用者可以在此介面中自由創建新的散點圖,或是隱藏和刪除一些不需要的散點圖,或者複製和重新調用所需的散點圖進行分析。同時,軟體支援各種不同種類的分析方法,包括:矩形圈選、多邊形圈選、十字象限分析法、水平和垂直方向線性門等。

在分析頁面可以用各種不同的組合呈現圖型和樣本,在分析頁面中,使用者可以建立多色直方圖的疊圖,列印多個圖型,比較樣本間的差異,使用放大工具擴大想看到的區域而不用調節電壓來設定想要呈現的道數範圍。

在分析頁面可以用各種不同的組合呈現圖型和樣本,在分析頁面中,使用者可以建立多色直方圖的疊圖,列印多個圖型,比較樣本間的差異,使用放大工具擴大想看到的區域而不用調節電壓來設定想要呈現的道數範圍。

BD Accuri C6 軟體可以輸出為FCS 3.0的格式以無縫地把客戶的數據輸入至其它流式細胞分析的軟體,包括 FCS Express™ and FlowJo™

Data collection

The Data Collection function lets users create new plots, hide or delete plots, or copy and re-use plots.

Data analysis

The Data Analysis function lets users create color overlay histograms, print multiple plots, compare samples, and create median statistics.

Statistics

The Statistics function lets users view information in a master table and easily copy and paste into spreadsheets.

Batch analysis
Batch Analysis

 
Sample figure creation using BD Accuri C6 software
Sample figure creation using BD Accuri C6 software

Embryonal carcinoma cell line 2102Ep was stained with antibodies corresponding to the proteins indicated on the histograms. Data was collected on a BD Accuri C6 cytometer. Data shows overlays of single-parameter plots after fluorescence compensation has been applied. Labeling on plots was added in presentation software after plots were dragged and dropped in from the BD Accuri C6 software file.

Stem cell marker comparisons facilitated with overlay plots
Stem cell marker comparisons facilitated with overlay plots

The human embryonal carcinoma (EC) cell line 2102Ep can serve as a reference standard for human embryonic and induced pluripotent stem cells because it expresses most of the same markers. After staining with antibodies to stem cell markers, 2102Ep EC cells were collected and analyzed on a BD Accuri C6. Overlays of single-parameter histograms were drawn with BD Accuri C6 software. Plots were dragged and dropped into presentation software, where labels were added. Note: The curve for SSEA-1 (gray in plot A), a negative pluripotency marker, is almost identical to the negative control (black).



Microsoft, PowerPoint, and Excel are registered trademarks of Microsoft Corporation.

FlowJo is a trademark of Tree Star, Inc.

FCS Express is a trademark of De Novo Software.