ELISA and ELISPOT Resources

Research Protocols

• Cytokine ELISA
• Mouse IgE ELISA Protocol
• Cytokine ELISPOT Reagent Set manual

BD OptEIA™ Set ELISA Techniques

1. Getting started   [ QuickTime ]   [ Windows Media ]
2. Capture antibody preparation and coating   [ QuickTime ]   [ Windows Media ]
3. Washing the plates   [ QuickTime ]   [ Windows Media ]
4. Standard preparation and handling   [ QuickTime ]   [ Windows Media ]
5. Blocking the plate   [ QuickTime ]   [ Windows Media ]
6. Standard and sample addition   [ QuickTime ]   [ Windows Media ]
7. Working detector preparation   [ QuickTime ]   [ Windows Media ]
8. TMB substrate preparation and addition   [ QuickTime ]   [ Windows Media ]
9. Stopping the reaction and reading the plate   [ QuickTime ]   [ Windows Media ]
10. Troubleshooting: Low/No Signal   [ QuickTime ]   [ Windows Media ]
11. Troubleshooting: High Background   [ QuickTime ]   [ Windows Media ]
12. Troubleshooting: Well-to-well Precision   [ QuickTime ]   [ Windows Media ]
13. Troubleshooting: Poor Standard Curve   [ QuickTime ]   [ Windows Media ]