Cell Culture Resources
Resources
Application Notes
Absorption, Transport and Permeability
- Maintenance and Functional Properties of Primary Turtle Bladder Epithelial Cells Cultured on BD Falcon™ Cell Culture Inserts
- Gamma-Glutamyl Transpeptidase Assay: An Example of a Protocol for Determining the Sidedness of Asymmetrical Expression of a Membrane Protein, Enzyme, or Transport Activity in an Epithelial or Other Cell Type
- Use of Automation-Compatible 24-well Insert System for Various
Cell-Based Assays - Comparison of the BD BioCoat™ Intestinal Epithelial Environment with the Conventional 21-Day Caco-2 System
- Use of the 3-Day BD BioCoat HTS Caco-2 Assay System for Compound Permeability Measurements
- Design and Evaluation of an Automation-Compatible Multiwell™ Insert for Cell-Based Assays
- Effects of ECM Proteins on Barrier Formation in Caco-2 Cells
- Morphological Comparison of Caco-2 Cells in the BD BioCoat Intestinal Epithelial Cell Environment and the Traditional 21-Day Caco-2 Culture System
- Optimization of Cell Culture Systems that Support Growth or Differentiation of Endothelial Cells
- In Vitro Study Of Cytokine-mediated Activation of Endothelial Cell Permeability Using BD Falcon Cell Culture Inserts
Angiogenesis
- Optimization of Cell Culture Systems that Support Growth or Differentiation of Endothelial Cells
- Methods for Implantation of BD Matrigel™ Matrix into Mice and Tissue Fixation
- An Image-Based Assay of Endothelial Cell Tube Formation as a Model of Angiogenesis
Blood Cell Chemotaxis and Migration
- Optimized Chemotaxis Conditions for Primary Blood Monocytes or THP-1 Cells Using BD Falcon™ FluoroBlok™ 96-Multiwell Insert Plates
- An In Vitro Assay for the Study of Neutrophil Migration Through Interstitial Matrix Using BD Falcon™ Cell Culture Inserts
- Use of Automation-Compatible 24-well Insert System for Various Cell-Based Assays
- Design and Evaluation of an Automation-Compatible Multiwell Insert for Cell-Based Assays
- Compatible Fluorophores and Dyes for BD Falcon Fluoroblok Inserts and Insert Systems
- Set Up Guidelines and Dimensional Templates for Fluorescence Plate Readers Used With BD Falcon HTS FluoroBlok Insert Systems and
BD BioCoat Multiwell Insert Cell-Based Assays
Bone Cell Culture
- Von Kossa Staining of Osteoclast Resorption on BD BioCoat Osteologic™ Discs
- Tartrate Resistant Acid Phosphatase (TRAP) Staining of Osteoclasts on BD BioCoat Osteologic Discs and MultiTest Slidess
- Chondrocyte Cell Culture and Tissue Engineering on BD BioCoat Osteologic Discs and BD™ 3D Calcium Phosphate Scaffolds
Cancer Research
- Set Up Guidelines and Dimensional Templates for Fluorescence Plate Readers Used With BD Falcon HTS FluoroBlok Insert Systems and BD BioCoat Multiwell Insert Cell-Based Assays
- Compatible Fluorophores and Dyes for BD Falcon Fluoroblok Inserts and Insert Systems
- Screening of Anti-Metastatic Compounds Using ZsGreen1 Reef Coral Fluorescent Protein (RCFP) Labeled HT-1080 Tumor Cells
- Screening of Anti-Metastatic Compounds by a Fluorescence Based Tumor Cell Invasion Assay
- BD Falcon HTS FluoroBlok 96-Multiwell Insert System Enhances High-Throughput Analysis of Cell-Based Assays
- Use of Automation-Compatible 24-well Insert System for Various Cell-Based Assays
- Design and Evaluation of an Automation-Compatible Multiwell Insert for Cell-Based Assays
- Methods for Implantation of BD Matrigel™ Matrix into Mice and Tissue Fixation
Cell Differentiation - General
- BD Falcon Cell Culture Inserts as a Support Substrate for an In Vitro Extracellular Matrix System
- Use of BD Falcon Cell Culture Inserts to Reconstruct a Differentiated Human Epidermis In Vitro: Expression of Cell Adhesion Molecules (Integrins)
- A Physiological and Morphological In Vitro Model For Normal Human Urothelium Cultured on BD Falcon Cell Culture Inserts
- In Vitro Study of Cytokine-Mediated Activation of Endothelial Cell Permeability Using BD Falcon™ Cell Culture Inserts
- Effects of ECM on Cell Proliferation and Differentiation of Hepatocytes
- Extracellular Matrix Proteins Influence Cell Morphology and Function in Rat Neural Cultures
- Chondrocyte Cell Culture and Tissue Engineering on BD BioCoat Osteologic Discs and BD™ 3D Calcium Phosphate Scaffolds
Cell Imaging
- Preparation of BD Falcon Cell Culture Inserts for Scanning Electron Microscopy
- Preparation of BD Falcon Cell Culture Inserts for Transmission Electron Microscopy
- Preparation of BD Falcon Cell Culture Inserts for Confocal Indirect Immunofluorescence: Fixation and Staining of Caco-2/bbe (C2) Cells with Various Dyes
- An Image-Based Assay of Endothelial Cell Tube Formation as a Model of Angiogenesis
- Quantitative High Content Analysis of Neurite Outgrowth
Cell Metabolism and Viability (oxygen consumption)
- Use of the BD™ Oxygen Biosensor System to Determine Doubling Time
- Calculating Oxygen Concentration from Fluorescence Data on the
BD Oxygen Biosensor System - Monitoring Adherent Cell Proliferation on BD Oxygen Biosensor Systems
- BD™ Oxygen Biosensor System Data Collection and Normalization
- BD™ Oxygen Biosensor System vs. Existing Methods of Measuring Toxicology
Co-Culture Systems
- Induction of Lymphoproliferation by Antigen-Primed Macrophages Across the BD Falcon Cell Culture Insert
- Use of BD Falcon Cell Culture Inserts to Evaluate Allelopathic Effects Among Marine Phytoplankters In Vitro
Drug Discovery
- Set Up Guidelines and Dimensional Templates for Fluorescence Plate Readers Used With BD Falcon FluoroBlok Insert Systems and BD BioCoat Multiwell Insert Cell-Based Assays
- Screening of Anti-Metastatic Compounds Using ZsGreen1 Reef Coral Fluorescent Protein (RCFP) Labeled HT-1080 Tumor Cells
- Screening of Anti-Metastatic Compounds by a Fluorescence Based Tumor Cell Invasion Assay
- The BD Falcon FluoroBlok 96-Multiwell Insert System Enhances High-Throughput Analysis of Cell-Based Assays
- Compatible Fluorophores and Dyes for BD Falcon FluoroBlok Inserts and Insert Systems
- Optimized Chemotaxis Conditions for Primary Blood Monocytes or THP-1 Cells Using BD Falcon FluoroBlok 96-Multiwell Insert Plates
- Heparin Preparations Immobilized to the BD™ Heparin Binding Plate
- BD PureCoat™ surfaces: Development of two novel culture surfaces that enhance cell performance in cell-based assays
General Cell Culture
- Advanced Cell Culture Surfaces: BD PureCoat surfaces provide improved cell attachment and growth of many cell types compared to standard tissue culture vessels
- Increased cell yields of LnCAP, BHK-21, and MRC-5 cells grown on
BD PureCoat surfaces
Neuroscience
- Extracellular Matrix Proteins Influence Cell Morphology and Function in Rat Neural Cultures
- Quantitative High Content Analysis of Neurite Outgrowth
- BD PureCoat Amine Supports Cell Attachment and Differentiation of Neural Cells
Stem Cell Biology
- Heparin Preparations Immobilized to the BD Heparin Binding Plate
- Migration of Human Mesenchymal Stem Cells using BD Falcon™ FluoroBlok™ Cell Culture Inserts
- BD PureCoat Surfaces Support Growth, Expansion, and Differentiation of Stem Cells
Three Dimensional Cell Culture
Brochures
Product Information Sheets
Protocols
Angiogenesis
- BD BioCoat Angiogenesis System Endothelial Cell Invasion
- BD BioCoat Angiogenesis System Endothelial Cell Migration
- BD BioCoat Angiogenesis System Endothelial Cell Tube Formation
- Methods for Implantation of BD Matrigel™ Matrix into Mice and Tissue Fixation
Blood Cell Chemotaxis and Migration
Cancer Research
- Methods for Implantation of BD Matrigel Matrix into Mice and Tissue Fixation
- BD BioCoat Tumor Invasion System 24-Multiwell
- BD BioCoat Tumor Invasion System 96-Multiwell
- BD BioCoat Matrigel Invasion Chamber
- Growth Factor Reduced Matrigel Invasion Chamber
Cell Differentiation - General
Drug Discovery
- BD BioCoat Angiogenesis System Endothelial Cell Invasion
- BD BioCoat Angiogenesis System Endothelial Cell Migration
- BD BioCoat Angiogenesis System Endothelial Cell Tube Formation
- BD BioCoat Tumor Invasion System 24-Multiwell
- BD BioCoat Tumor Invasion System 96-Multiwell
- Heparin Preparations Immobilized to the BD Heparin Binding Plate
Stem Cells
Three Dimensional Culture
Tools
- 96-well Plate Grid (helps locate wells on your 96-well ELISA plate)
- Cell Record Worksheet (a useful tool to help organize information on the cell types used in your lab)
- Rotor/Adapter Selection Guide (pick the right rotor/adapter for your tube and application)
- Centrifuge Tube Chemical Resistance Chart (pick the tube material that best fits your needs)
Cell Culture