Cell Culture Resources
Application Notes
Absorption, Transport and Permeability
- Maintenance and Functional Properties of Primary Turtle Bladder Epithelial Cells Cultured on BD Falcon™ Cell Culture Inserts
- Gamma-Glutamyl Transpeptidase Assay: An Example of a Protocol for Determining the Sidedness of Asymmetrical Expression of a Membrane Protein, Enzyme, or Transport Activity in an Epithelial or Other Cell Type
- Use of Automation-Compatible 24-well Insert System for Various Cell-Based Assays
- Comparison of the BD BioCoat™ Intestinal Epithelial Environment with the Conventional 21-day Caco-2 System
- Use of the 3-Day BD BioCoat™ HTS Caco-2 Assay System for Compound Permeability Measurements
- Design and Evaluation of an Automation-Compatible Multiwell Insert for Cell-Based Assays
- Effects of ECM Proteins on Barrier Formation in Caco-2 Cells
- Morphological Comparison of Caco-2 Cells in the BD BioCoat™ Intestinal Epithelial Cell Environment and the Traditional 21-Day Caco-2 Culture System
- Optimization of Cell Culture Systems that Support Growth or Differentiation of Endothelial Cells
Angiogenesis
Blood Cell Chemotaxis and Migration
- Optimized Chemotaxis Conditions for Primary Blood Monocytes or THP-1 Cells Using BD Falcon™ FluoroBlok™ 96-Multiwell Insert Plates
- An In Vitro Assay for the Study of Neutrophil Migration Through Interstitial Matrix Using BD Falcon™ Cell Culture Inserts
- Use of Automation-Compatible 24-well Insert System for Various Cell-Based Assays
- Design and Evaluation of an Automation-Compatible Multiwell Insert for Cell-Based Assays
- Compatible Fluorophores and Dyes for BD Falcon™ Fluoroblok™ Inserts and Insert Systems
- Set Up Guidelines and Dimensional Templates for Fluorescence Plate Readers Used With BD Falcon™ HTS FluoroBlok™ Insert Systems and BD BioCoat™ Multiwell Insert Cell-Based Assays
Bone Cell Culture
- Von Kossa Staining of Osteoclast Resorption on BD BioCoat Osteologic Discs
- Tartrate Resistant Acid Phosphatase (TRAP) Staining of Osteoclasts on BD BioCoat™ Osteologic Discs and MultiTest Slidess
- Chondrocyte Cell Culture and Tissue Engineering on BD BioCoat™ Osteologic Discs and BD™ 3D Calcium Phosphate Scaffolds
Cancer Research
- Set Up Guidelines and Dimensional Templates for Fluorescence Plate Readers Used With BD Falcon™ HTS FluoroBlok™ Insert Systems and BD BioCoat™ Multiwell Insert Cell-Based Assays
- Compatible Fluorophores and Dyes for BD Falcon™ Fluoroblok™ Inserts and Insert Systems
- Screening of Anti-Metastatic Compounds Using ZsGreen1 Reef Coral Fluorescent Protein (RCFP) Labeled HT-1080 Tumor Cells
- Screening of Anti-Metastatic Compounds by a Fluorescence Based Tumor Cell Invasion Assay
- BD Falcon™ HTS FluoroBlok™ 96-Multiwell Insert System Enhances High-Throughput Analysis of Cell-Based Assays
- Use of Automation-Compatible 24-well Insert System for Various Cell-Based Assays
- Design and Evaluation of an Automation-Compatible Multiwell Insert for Cell-Based Assays
- Methods for Implantation of BD Matrigel™ Matrix into Mice and Tissue Fixation
Cell Differentiation – General
- BD Falcon™ Cell Culture Inserts as a Support Substrate for an In Vitro Extracellular Matrix System
- Use of BD Falcon™ Cell Culture Inserts to Reconstruct a Differentiated Human Epidermis In Vitro: Expression of Cell Adhesion Molecules (Integrins)
- A Physiological and Morphological In Vitro Model For Normal Human Urothelium Cultured on BD Falcon™ Cell Culture Inserts
- In Vitro Study of Cytokine-Mediated Activation of Endothelial Cell Permeability Using BD Falcon™ Cell Culture Inserts
- Effects of ECM on Cell Proliferation and Differentiation of Hepatocytes
- Extracellular Matrix Proteins Influence Cell Morphology and Function in Rat Neural Cultures
Cell Imaging
- Preparation of BD Falcon™ Cell Culture Inserts for Scanning Electron Microscopy
- Preparation of BD Falcon™ Cell Culture Inserts for Transmission Electron Microscopy
- Preparation of BD Falcon™ Cell Culture Inserts for Confocal Indirect Immunofluorescence: Fixation and Staining of Caco-2/bbe (C2) Cells with Various Dyes
- An Image-Based Assay of Endothelial Cell Tube Formation as a Model of Angiogenesis
- Quantitative High Content Analysis of Neurite Outgrowth
Cell Metabolism and Viability (oxygen consumption)
- Use of the BD™ Oxygen Biosensor System to Determine Doubling Time
- Calculating Oxygen Concentration from Fluorescence Data on the BD™ Oxygen Biosensor System
- Monitoring Adherent Cell Proliferation on BD™ Oxygen Biosensor Systems
- BD™ Oxygen Biosensor System Data Collection and Normalization
- BD™ Oxygen Biosensor System vs. Existing Methods of Measuring Toxicology
Co-Culture Systems
Drug Discovery
- Set Up Guidelines and Dimensional Templates for Fluorescence Plate Readers Used With BD Falcon™ HTS FluoroBlok™ Insert Systems and BD BioCoat™ Multiwell Insert Cell-Based Assays
- Screening of Anti-Metastatic Compounds Using ZsGreen1 Reef Coral Fluorescent Protein (RCFP) Labeled HT-1080 Tumor Cells
- Screening of Anti-Metastatic Compounds by a Fluorescence Based Tumor Cell Invasion Assay
- BD Falcon™ HTS FluoroBlok™ 96-Multiwell Insert System Enhances High-Throughput Analysis of Cell-Based Assays
- Compatible Fluorophores and Dyes for BD Falcon™ Fluoroblok™ Inserts and Insert Systems
- Optimized Chemotaxis Conditions for Primary Blood Monocytes or THP-1 Cells Using BD Falcon™ FluoroBlok™ 96-Multiwell Insert Plates
- Heparin Preparations Immobilized to the BD™ Heparin Binding Plate
- BD PureCoat Surfaces: Development of Two Novel Culture Surfaces that Enhance Cell Performance in Cell-Based Assays
Neuroscience
Stem Cell Biology
Three Dimensional Cell Culture
Protocols
Angiogenesis
Blood Cell Chemotaxis and Migration
Cancer Research
Cell Differentiation - General
Drug Discovery
- BD BioCoat™ Angiogenesis System Endothelial Cell Invasion
- BD BioCoat™ Angiogenesis System Endothelial Cell Migration
- BD BioCoat™ Angiogenesis System Endothelial Cell Tube Formation
- BD BioCoat™ Tumor Invasion System 24 Multiwell
- BD BioCoat™ Tumor Invasion System 96 Multiwell
- Heparin Preparations Immobilized to the BD™ Heparin Binding Plate
Stem Cells
Three Dimensional Culture
Tools
- 96-well Plate Grid (helps locate wells on your 96-well ELISA plate)
- Cell Record Worksheet (a useful tool to help organize information on the cell types used in your lab)
- Rotor/Adapter Selection Guide (pick the right rotor/adapter for your tube and application)
- Centrifuge Tube Chemical Resistance Chart (pick the tube material that best fits your needs)
- ThermoPlastics Properties Chart (determine which material offers the right properties for your lab's protocols)
- Nomograph for RCF Determination (Calculating Relative Centrifugal Force RCF from Revolutions Per Minute RPM)