ADME Resources

ADME Support Documents

Drug Permeability

• Application Notes

  • Automation of Pre-coated PAMPA Plates Improves Predictability, Reproducibility, and Efficiency
  • A Novel Design of Artificial Membrane for Improving the PAMPA Model
  • Use of Automation-Compatible 24-Well Insert System for Various Cell-Based Assays
  • Comparison of the BD BioCoat™ Intestinal Epithelial Environment with the Conventional 21-Day Caco-2 System
  • Use of the 3-Day BD BioCoat HTS Caco-2 Assay System for Compound Permeability Measurements
  • Design and Evaluation of an Automation-Compatible Multiwell™ Insert for Cell-Based Assays

• Protocols

  • Lucifer Yellow Permeability Assay Using BD Falcon™ HTS 96-Multiwell Insert Systems
  • Preparation of 21-Day Caco-2 Cell Monolayers for BD Falcon™ 96-Multiwell Insert Systems
  • Preparation of 21-Day Caco-2 Cell Monolayers for BD Falcon™ 96-Multiwell Insert Systems

• Tools

  • BD Falcon HTS 96 Multiwell™ Insert Systems
  • BD Falcon HTS 96 Multiwell™ Insert Systems
  • Product Use Guidelines for Automated or Manual 24-Multiwell Insert Systems

Drug Transport

• Application Notes

  • Cloning and Characterization of Cynomolgus Monkey and Rhesus Monkey Organic Anion Transporting Polypeptide 1B3, and Comparison of Their Function with Human OATP1B3

• Protocols

  • Drug Transport Assay for BD Falcon™ 24-Multiwell Insert Systems

• Tools

  • BD Falcon HTS Multiwell™ Insert Systems
  • BD Falcon HTS Multiwell™ Insert Systems
  • Product Use Guidelines for Automated or Manual 24-Multiwell Insert Systems

Cytochrome P450 Inhibition

• Application Notes

  • Time-Dependent Inhibition (TDI) of Human Cytochrome P450: Evaluation of Screening Methods Utilizing RapidFire® MS Technology
  • Differential Time- and NADPH-dependent inhibition of CYP2C19 by enantiomers of fluoxetine
  • Validation of GLP Time-Dependent Cytochrome P450 Inhibition Assays with Assays with LC/MS Detection

Cytochrome P450 Metabolism

• Tools

  • Enzyme Assays, CYP2C8: Assay Conditions for Paclitaxel 6(alpha)-Hydroxylation
  • Enzyme Assays, CYP2C9: Assay Conditions for Diclofenac 4’-Hydroxylation
  • Enzyme Assays, CYP2D6: Assay Conditions for Bufuralol 1’-Hydroxylation

Reaction Phenotyping

• Application Notes

  • Glucuronidation of (R,R)-Formoterol and (S,S)-Formoterol is Catalyzed by cDNA-expressed Human Uridine 5´-Diphospho-Glucuronosyltransferases and Human Liver Microsomes

Hepatocyte Culture and Metabolism

• Application Notes

  • BD Matrigel™ Matrix Thin-Coat and BD Matrigel™ Matrix Overlay Improved CYP450 Activities in Human Cryopreserved Hepatocytes

• Protocols

  • Determination of Hepatocyte Cell Concentration, Recovery and Viability
  • BD Gentest™ CyroHepatocytes Percoll™ Recovery Method
  • Plating of BD Gentest™ Inducible-Qualified Human CyroHepatocytes

non-P450 Metabolism

• Application Notes

  • Expression and Characterization of Recombinant Human Carboxylesterases 1 and 2 Expreessed in Baculovirus-infected High Five Insect Cells

Protein Binding

• Application Notes

  • Validation of an Automated High Throughput Plasma Protein Binding Assay 

UGT Metabolism

• Application Notes

  • Glucuronidation of (R,R)-Formoterol and (S,S)-Formoterol is Catalyzed by cDNA-expressed Human Uridine 5´-Diphospho-Glucuronosyltransferases and Human Liver Microsomes

• Protocol

  • Mammalian Liver Cytosol
  • Mammalian Liver Microsomes
  • Mammalian Liver S9
  • BD Supersomes™ cDNA-Expressed Cytochrome P450 Enzymes
  • BD Supersomes™ cDNA-Expressed Flavin Monooxygenase (FMO) Enzymes
  • BD Supersomes™ cDNA-Expressed N-Acetyltransferase (NAT) Enzymes
  • BD Supersomes™ cDNA-Expressed Monoamine Oxidase (MAO) Enzymes
  • BD Supersomes™ cDNA-Expressed UDP-Glucuronosyl Transferase Enzymes

Immunoblotting and Immunoinhibition

• Tools

  • Antibody Specificity Tables: Human FMO Immunoblotting Antibodies
  • Antibody Specificity Tables: Human P450 Immunoblotting Antibodies
  • Antibody Specificity Tables: Human P450 Inhibitory Antibodies
  • Antibody Specificity Tables: Human UGT Immunoblotting Antibodies
  • Antibody Specificity Tables: Rat P450 Immunoblotting Antibodies