Cell Analysis and Reagents

Immunogenicity of biological therapeutics

The Custom Technology Team specializes in developing novel multiplexed protein assays for immune monitoring. These assays have been designed to measure humoral immune response to biological therapeutics and vaccine antigens, vaccine carrier proteins, as well as infection.

CTT uses the BD™ Cytometric Bead Array (CBA) as an assay platform for immune monitoring. This bead-based flow cytometry application allows users to quantify multiple proteins simultaneously. CTT has extensive experience working with pharmaceutical companies developing CBA-based antibody detection assays.

Intracellular cytokine assays

CTT offers flow cytometry-based intracellular cytokine assays and sample processing services for immune monitoring relevant for studies of autoimmune disease, HIV and tumor vaccine development, allergy, and viral and infectious diseases. These custom, premixed cytokine assays speed immune monitoring by providing data on multiple cytokines simultaneously on specific cellular subsets in complex samples. CTT’s intracellular cytokine assays also save precious sample, using only 0.5 mL of blood for multiple cytokine results.

Custom, premixed CBA kits

To help improve lab efficiency and standardization, CTT can provide researchers with premixed formulations of off-the-shelf CBA reagents and detection beads. CBA reagents are available for the detection of human, mouse, or rat cytokines and chemokines and growth factors from serum, plasma, or tissue culture supernatant samples.

A custom CBA assay designed by CTT to detect anti-epoetin alfa

A custom CBA assay designed by CTT to detect anti-epoetin alfa

A multiplexed protein assay designed to detect antibodies to epoetin alfa using four beads of distinct fluorescence intensity coated with epoetin alfa (EPO), sperm whale myoglobin (SWM, a negative control), or human IgG (IgG, a positive control). A fourth bead was left blank and served as a reference bead control.

Ferbas J, Thomas J, Hodgson J, Gaur A, Casadevall N, Swanson SJ. Feasibility of a multiplex flow cytometric bead immunoassay for detection of anti-epoetin alfa antibodies. Clin Vaccine Immunol. 2007;14:1165-1172.

ELISPOT services

ELISPOT services

CTT’s ELISPOT assay services allow researchers to monitor cellular immune response to various antigens. Shown here is an ELISPOT assay for human IL-5 production demonstrating a response to stimulation with cytomegalovirus antigens from a lysate (CMV-L) or CMV peptide mix (CMV-P) (shown in triplicate). Staphylococcal enterotoxin B (SEB) serves as a positive control. Additionally, CTT actively participates in an NIH-sponsored program to monitor interlaboratory standardization protocols for the ELISPOT and Colony Forming Cell assays, involving more than 14 laboratories worldwide.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.