T Cell Immunology
Tools
BD Phosflow™ technology offers an extremely powerful means of obtaining kinetic information on T-cell subset-specific signaling processes.
T cells are activated and regulated by complex pathways involving a number of signal transduction molecules, including receptors for antigens and cytokines, kinases, and transcription factors. Signals involving protein phosphorylation play a role in the differentiation of naïve CD4+ T cells into Th1, Th2, Th9, Th17, Tfh, or Treg cells.Detecting transient phosphorylation events: BD Phosflow technology
Innovative BD Phosflow technology is the first complete flow cytometry solution to reveal intracellular data on basal and induced protein phosphorylation events in both cell lines and primary cells. The BD Phosflow approach is especially informative for studying T cells, in which phosphorylation of signaling proteins leads to the expression of particular T-cell phenotypes.
Specialized antibodies, buffers, and kits
BD provides reagents and kits for the study of protein phosphorylation by flow cytometry. Developed collaboratively by BD and researchers at Stanford University, BD Phosflow technology consists of phosphoprotein-specific, fluorochrome-labeled monoclonal antibodies, along with a system of optimized buffers that fix the cellular proteins to maintain their phosphorylation state and prepare cells for antibody staining.
Researchers can use the BD Phosflow system in combination with cell surface markers to reliably determine the level of key phosphorylated signaling proteins from populations within heterogenous samples such as whole blood. Even rare cell subtypes can be identified without upfront enrichment.
