T-Cell Immunology
Sample Data
Flow cytometric analysis of PE anti-human IL-17A on stimulated PBMCs
Human PBMCs were stimulated with PMA/Ionomycin in the presence of BD GolgiStop™ protein transport inhibitor (Cat. No. 554724) for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm™ reagents (Cat. No. 554714) followed by staining with PE anti-human IL-17A (Cat. No. 560486), and Alexa Fluor® 647 anti-human IFN-γ (Cat. No. 557729; left panel) or APC anti-human IL-4 (Cat. No. 554486; right panel). The dot plots were derived from a CD4+ lymphocyte gate. Flow cytometry was performed using a BD FACSCalibur™ system.
Measurement of Th17 cultures using the BD CBA Th1/Th2/Th17 Kits
A) CD4+ human memory T cells isolated from whole blood were stimulated with plate-bound anti-CD3 and soluble anti-CD28 alone (blue) or in the presence of recombinant IL-23 (orange).
B) Naïve CD4+ splenocytes were stimulated with plate-bound anti-CD3 and soluble anti-CD28 alone (blue) or in the presence of recombinant TGF-β and IL-6 (orange).
