Stem Cell Research
Sample Data
To allow researchers more time for experiments, BD, in collaboration with Stem Cell Technologies and WiCell™ Research Institute, provides a complete, pre-qualified environment for culturing hESCs. The environment is composed of BD Matrigel™ hESC-qualified matrix and mTeSR™1, a completely defined medium. For human embryonic stem cell (hESC) studies requiring more defined culture conditions, BD™ Laminin/Entactin Complex High Concentration allows you to use purified extracellular matrix proteins with a defined medium to culture pluripotent hESCs.
Embryonic stem cell cultured on BD Matrigel matrix
Brightfield image of H9 hESCs cultured in mTeSR™1 maintenance medium (StemCell Technologies) on BD Matrigel hESC-qualified matrix (Cat. No. 354277) coated plates.
Bioimaging of hESCs cultured on BD Matrigel matrix
H9 hESCs were cultured in mTeSR™1 maintenance medium on BD Falcon™ 96-well imaging plates (Cat. No.353219) that were coated with BD Matrigel hESC-qualified matrix (Cat. No. 354277). Cells were fixed with 4% paraformaldehyde, followed by BD Perm/Wash™ buffer (Cat.No. 554723). Multicolor cell staining was performed by incubating cells with the following fluorochrome-conjugated antibodies: Sox2 Alexa Fluor® 647 (Cat. No. 560302) pseudocolored yellow, Oct3/4 Alexa Fluor® 555 (Cat. No. 560306) pseudocolored red, and SSEA-4 Alexa Fluor® 488 (Cat. No. 560308) pseudocolored green. Cell nuclei were counterstained using Hoechst 33342 pseudocolored blue. The cells were imaged on a BD Pathway™ 435 bioimager using a 10x objective.
Flow cytometry analysis of hESCs cultured on BD Matrigel matrix
Flow cytometry data from H9 cells grown in 6-well culture plates coated with BD Matrigel hESC-qualified matrix and mTeSR™1 medium. Cells were dissociated into a single-cell suspension and then fixed with 4% paraformaldehyde, followed by BD Perm/Wash buffer. Cells were incubated with the same cocktail of fluorchrome-conjugated antibodies used in the imaging experiments described above except that Oct3/4 PE (Cat. No. 560186) was used in place of Oct3/4 Alexa Fluor® 555. Cells were run on a BD™ LSR II cell analyzer, and data was analyzed using BD FACSDiva™ software.
For human embryonic stem cell (hESC) studies requiring more defined culture conditions, BD™ Laminin/Entactin Complex High Concentration allows you to use purified extracellular matrix proteins with a defined medium to culture pluripotent hESCs.
hESCS cultured on BD Laminin/Entactin Complex High Concentration
A) Phase contrast images of H9 cells grown on BD Laminin/Entactin Complex High Concentration (Cat No. 354259) coated plates in mTeSR™1 maintenance medium. The image was captured at 4x magnification.
hESCS cultured on BD Laminin/Entactin Complex High Concentration
B) Flow cytometry analysis of H9 hESCs cultured on a BD Laminin/Entactin Complex High Concentration coated surface in mTeSR™1 maintenance medium; left) 95% of cells stained positive for Oct3/4 (green), and right) 91% of cells stained positive for SSEA-4 (green). The isotype control is shown in red.
