Intracellular Flow Cytometry

Multiparameter analysis of cytokine, transcription factor, and phosphoprotein expression by flow cytometry.

For some cell types, such as Th17 and regulatory T cells (Tregs), definitive identification depends on the combined use of surface and intracellular markers such as cytokines or transcription factors.

Intracellular flow cytometry provides rich information concerning cellular function and signaling responses. Fluorescent antibodies specific for cell surface markers can be combined with markers of apoptosis, proliferation, and protein phosphorylation to determine which cell subsets respond to various stimuli or treatments. The combined use of multiple markers decreases data acquisition time and conserves precious samples, since more parameters can be measured on a per-cell basis.

Product List

Product List

Fluorochrome-conjugated antibodies, buffers, kits, and protocols to facilitate intracellular flow cytometry. BD antibodies are tested in biologically relevant model systems. These established tools enable new discoveries in fields such as immunology, inflammation, and stem cell biology.

Techniques

An overview of general techniques for intracellular flow cytometry and of specific methods for detection of cytokines and inflammatory mediators, transcription factors, phosphoproteins, and combinations of these target molecules.


For Research Use Only. Not for use in diagnostic or therapeutic procedures.