Intracellular Flow Cytometry
Multiparameter analysis of cytokine, transcription factor, and phosphoprotein expression by flow cytometry.
For some cell types, such as Th17 and regulatory T cells (Tregs), definitive identification depends on the combined use of surface and intracellular markers such as cytokines or transcription factors.
Intracellular flow cytometry provides rich information concerning cellular function and signaling responses. Fluorescent antibodies specific for cell surface markers can be combined with markers of apoptosis, proliferation, and protein phosphorylation to determine which cell subsets respond to various stimuli or treatments. The combined use of multiple markers decreases data acquisition time and conserves precious samples, since more parameters can be measured on a per-cell basis.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.