Bead-Based Immunoassays

BD CBA General Workflow

Each capture bead in the array has a unique fluorescence intensity and is coated with a capture antibody specific for a single analyte.
A combination of different beads is mixed with a sample or standard and a mixture of detection antibodies that are conjugated to
a reporter molecule (PE). Following incubation and subsequent washing, the samples are acquired on a flow cytometer.

The FCAP Array analysis software gates on each individual bead population and determines the median fluorescence intensity (MFI) for each analyte in the array. It generates a standard curve and performs interpolation of sample concentrations compared to the standard curve and generates an analysis report in tabular format.