Bead-Based Immunoassays

Multiplexed quantitation and detection

BD™ Cytometric Bead Array (CBA) is a flow cytometry application that allows users to quantify multiple proteins simultaneously. The BD CBA system uses the broad dynamic range of fluorescence detection offered by flow cytometry and antibody-coated beads to efficiently capture analytes. Each bead in the array has a unique fluorescence intensity so that beads can be mixed and run simultaneously in a single tube. This method significantly reduces sample requirements and time to results in comparison with traditional ELISA and Western blot techniques.

BD CBA Flex Sets provide an open and configurable method of detection, so that researchers can build their own multiplexes.

BD CBA Kits are preconfigured for achieving consistent results for routine panels.

Product List

Product List

The BD CBA portfolio includes assays for measurement of a variety of soluble and intracellular proteins including cytokines, chemokines, growth factors, and phosphorylated cell signaling proteins.

Workflow

Workflow

Each capture bead in a BD CBA kit has been conjugated with a specific antibody. The detection reagents provided in the kit are phycoerythrin (PE)-conjugated antibodies, which provide a fluorescent signal in proportion to the amount of bound analyte.


For Research Use Only. Not for use in diagnostic or therapeutic procedures.